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Life Science > Molecular Biology > Whole Genome Amplification > Formalin Fixed Paraffin Embedded DNA Amplification
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Whole Genome Amplification
Formalin Fixed Paraffin Embedded DNA Amplification | ||
| Too many samples? Now available: WGA and WTA Services |
DNA isolated from archived tissue is often of poor quality making it unsuitable for most commercially available whole genome amplification kits that require high quality starting material. The flexibility of the GenomePlex technology enables the use of compromised starting material, such as DNA isolated from FFPE tissue, for whole genome amplification. Sigma offers products for the purification of DNA from FFPE tissue (G1N10) and subsequent whole genome amplification (WGA2). We also offer the GenomePlex Tissue WGA Kit (WGA5) which includes optimized reagents for tissue disruption and cell lysis eliminating the need for tedious organic extractions to remove excess paraffin or DNA purification prior to amplification.
White Papers
Technical Articles
Posters
- qPCR Quality Assessment of Whole Genome Amplified FFPE DNA Samples and their Use on a BAC aCGH Platform (752 Kb PDF)
- Array CGH Analysis of Challenging Samples (210 Kb PDF)
Protocols
Extraction
Amplification
- GenomePlex Complete Whole Genome Amplification Kit (Product No. WGA2)
- GenomePlex Tissue Whole Genome Amplification Kit (Product No. WGA5)
Reamplification and purification
FFPE Diagnostic Protocol (257 Kb PDF)
Publications:
Click on the title to view the abstract on PubMed.
| Title | Author / Journal |
| Evaluation of whole genome amplification protocols for array and oligonucleotide CGH. | Hittelman A, et al.Diagn Mol Pathol. 2007 Dec; 16(4), 198-206. |
| Application of array CGH on archival formalin-fixed paraffin embedded tissues including small numbers of microdissected cells. | Johnson, N. A. et al. Lab. Invest. 86(9), 968-78 (2006). |
| Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. | Little, S. E. et al. Genomics Oct. 31, 2005. |
Webcast:
 Advances in Genetic Analyis of Archived Samples, addresses the recent advances in Whole Genome Amplification that have made it possible to gain access to sufficient quantities of DNA from the millions of archived samples to perform a number of downstream applications including QPCR, CGH microarray, STR analysis and SNP analysis. |
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