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Whole Genome Amplification

Whole Genome Amplification Sequencing Technology (SEQXE)

The SeqPlex Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful NGS library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow.

This kit is an extension of the WGA product line and has been developed to integrate into the Illumina® (GAIIx sequencer), SOLiD™ System (from life technologies), Ion Torrent (from life technologies) and other next generation sequencing workflows, as a DNA amplification technology.

Features and Benefits

  • Compatible with next generation sequencing platforms
  • Patent pending random priming technology amplifies fragmented or intact DNA (obtained by ChIP or any other protocol)
  • Provides highly uniform amplification across the entire genome with minimal sequence bias
  • Directly compatible with validated sequencing library preparation protocols on existing NGS platforms
  • Enhanced primer design for more complete genomic coverage

Workflow

WGAseq Flow Chart

Application Data


SeqPlex Enhanced DNA Amplification (SEQXE) was performed on 100 picograms of ChIP DNA from a pool prepared using Imprint® Ultra Chromatin Immunoprecipitation Kit (CHP2) and 4.32 micrograms of H1.4K26me2 (H8289, Lot 87k4784) antibody on cross-linked, fragmented chromatin (200-500bp) from 4x107 DU145 cells. Approximately 150 ng of SeqPlex product or original ChIP DNA were submitted for Illumina sequencing. Standard Sequencing Library Preparation for ChIP DNA was used prior to sequencing on an Illumina GAIIx sequencer. Sequence alignments were visualized with Integrated Genome Browser 6.5.3.

97% of nucleotides with >8x reads aligned are found in both SeqPlex ChIP and Unamplified ChIP samples. Reads were analyzed using 400 bp floating windows.

SeqPlex (bottom) and Unamplified (top) show similar sequence alignment. Chromosome 7 reads were visualized using Integrated Genome Browser 6.5.3.

Order Information

Product No. Description Quantity Add to Cart
SEQXE-10RXN SeqPlex Enhanced DNA Amplification Kit 10 Reactions
SEQXE-50RXN SeqPlex Enhanced DNA Amplification Kit 50 Reactions
SEQXE-500RXN SeqPlex Enhanced DNA Amplification Kit 500 Reactions