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Unlock the Potential within a Single Cell
The GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) is designed to amplify the genome of a single cell resulting in a million-fold amplification yielding microgram quantities of genomic DNA. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. However, the GenomePlex® technology provides amplification efficiency with WGA DNA Polymerase. The Single Cell WGA kit includes all of the reagents necessary for cell lysis and subsequent whole genome amplification.
The Single Cell WGA kit has been validated with numerous downstream applications and is suitable for amplification from a variety of sources including: plant, polycarbonate fixed cells, lymphocytes, renal cancer cells, epithelial cells, leukemia cells, and fibroblast amniotic cells. The amplified genome is compatible with many downstream applications including: comparative genomic hybridization (CGH), microsatellite/STR analysis, SNP analysis, QPCR, and other genotyping analysis.
Features & Benefits
- Abundant yields of DNA generated from single cell genomic DNA in less than four hours
- Accurate representation of entire genome
- Identification of genome-wide copy number and chromosomal abnormalities
- No detectable amplification product for the negative control
Application Data
Technical Papers
Whole Genome Amplification for Single Cell Biology
Single Cell WGA Posters
GenomePlex® Single Cell WGA Kit Testimonials
Ordering Information
| Product Code |
Product Name |
Technical Bulletin |
| WGA4 |
GenomePlex® Single Cell Whole Genome Amplification (WGA) Kit |
 |
GenomePlex® WGA kits are not for use for third party services or diagnostic applications. For information regarding services for whole genome amplification or whole transcriptome amplification of population, clinical samples, or diagnostics applications, contact Rubicon Genomics (www.rubicongenomics.com).
References
Barker, D.L., et al. Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linage panel. Genomic Research, 14, 901-7 (2004).
Gribble, S., et al. Chromosome paints from single copies of chromosomes. Chromosome Research, 12, 143-51 (2004).
GenomePlex and OmniPlex are Registered Trademarks of Rubicon Genomics. This product is for research use only. Use of this product for human or animal therapeutic or diagnostic purposes requires licensing from Rubicon Genomics, Inc. Unauthorized use violates proprietary rights of Rubicon Genomics, Inc (www.rubicongenomics.com).
TaqMan® is a registered trademark of Roche Molecular Systems, Inc.
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