Whole Genome Amplification

The GenomePlex® Whole Genome Amplification (WGA) family of products provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out.
The GenomePlex WGA product line utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex® Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles.
WGA has been used in a variety of applications,1, 2, 3 and is suitable for use with purified genomic DNA from a variety of sources. GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5 to 10 μg of WGA product. After purification, the WGA product can be analyzed in a manner similar to any genomic or chromosomal DNA sample.

  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil and formalin-fixed paraffin-embedded (FFPE) tissue
  • See a complete representation of the entire genome with no detectable allele bias.
  • Increase amplification accuracy with WGA DNA Polymerase
  • Preserve precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500fold) in less than three hours
  • Enjoy the compatibility GenomePlex provides with many downstream applications: TaqMan® assays, CGH analysis, SNP analysis, sequencing, or store at 20 °C for future use.

References:
1. Little, S.E., et al., Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics. Oct 31, 2005.
2. Barker, D. L., et al., Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel. Genome Res., 14, 901-907 (2004).
3. Gribble, S., et al., Chromosome paints from single copies of chromosomes. Chromosome Res., 12, 143-151 (2004).
4. Thorstenson, Y. R., et al., An Automated Hydrodynamic Process for Controlled, Unbiased DNA Shearing. Genome Res., 8, 848-855 (1998).
5. Park, J.W., et al., Comparing Whole-Genome Amplification Methods and Sources of Biological Samples for Single-Nucleotide Polymorphism Genotyping. Clinical Chemistry., 51(8), 1520-1523 (2004).

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GE25-6600-20 Genomiphi Hy Kit GE Healthcare, 25-6600-20, sufficient for 100 reactions
GE25-6600-30 GenomiPhi V2 DNA Amplification Kit, 25 RXN GE Healthcare, 25-6600-30, sufficient for 25 reactions
GE25-6600-31 Genomiphi V2 DNA Amplification Kit GE Healthcare, 25-6600-31, sufficient for 100 reactions
GE25-6600-32 Genomiphi V2 DNA Amplification Kit GE Healthcare, 25-6600-32, sufficient for 500 reactions
GE25-6601-24 RTG GenomiPhi V3 DNA Amplification Kit, 24 RXN GE Healthcare, 25-6601-24, sufficient for 24 reactions
GE25-6601-97 RTG GenomiPhi V3 DNA Amplification Kit, 480 RXN GE Healthcare, 25-6601-97, sufficient for 480 reactions
GE25-6603-24 RTG GenomiPhi HY DNA Amplification Kit, 24 RXN GE Healthcare, 25-6603-24, sufficient for 24 reactions
GE25-6603-97 RTG GenomiPhi HY DNA Amplification Kit, 480 RXN GE Healthcare, 25-6603-97, sufficient for 480 reactions
WGA1 GenomePlex® Whole Genome Amplification (WGA) Kit Kit for whole genome amplification from a variety of DNA sources including FFPE tissue
WGA2 GenomePlex® Complete Whole Genome Amplification (WGA) Kit Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
WGA3 GenomePlex® WGA Reamplification Kit Reamplification of WGA product with minimal bias
WGA4 GenomePlex® Single Cell Whole Genome Amplification Kit Amplify genome of a single cell