PCR

WGA Protocols

Whole Genome Amplification (WGA-1) Protocols

Sigma-Aldrich is dedicated to providing the most recent technical content to support our customer's scientific research. Our R&D group works diligently to provide protocols and application data that promotes scientific discovery, as well as supporting the success of customers. Visit this site often for the most recent whole genome amplification data and protocol updates.

 


Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases. Since the archived DNA is precious material and usually available in limited quantities, amplification of the samples is essential. However, amplifying the FFPE tissue can be a difficult task due to the damaged template that results from the archiving process. This protocol provides a convenient method to amplify and purify genomic DNA from FFPE tissue. The methods described are completed using one of the commercially available kits for extracting DNA from FFPE tissue, the GenomePlex® WGA kit, and GenElute™ PCR Clean-up Kit.

 


This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Samples that result in low quantity genomic DNA can hinder the researcher’s ability to perform downstream analysis. The protocol presents a simple and accurate tool to immortalize DNA for downstream applications. This method describes the means for amplifying nanogram amounts of starting genomic DNA with little or no detectable bias resulting in microgram quantities. The protocol is completed using the GenElute™ Mammalian Genomic DNA MiniPrep Kit, GenomePlex® WGA kit, and GenElute™ PCR Clean-up Kit.

 


Blood cards provide the convenience of archiving small volumes of blood. Many times genomic DNA from samples can have low yield. This quantity can hinder the researcher's ability to perform downstream analysis. This method describes amplifying nanogram amounts of starting genomic DNA with little or no detectable bias resulting in microgram quantities. In addition, the protocol provides a simple and convenient method to extract the genomic DNA from the blood card, amplify the genomic DNA, and rapidly purify the PCR amplified products. The methods described are completed using the GenElute™ Blood Genomic DNA Kit, GenomePlex® WGA kit, and GenElute™ PCR Clean-up Kit.

 


Whole blood is a common source of material when performing genetic analysis. Many times genomic DNA from samples can have low yield. This quantity can hinder the researcher's ability to perform downstream analysis. This method describes amplifying nanogram amounts of starting genomic DNA with little or no detectable bias resulting in microgram quantities. In addition, the protocol provides a simple and convenient method to isolate pure genomic DNA from fresh or aged whole blood, amplify the genomic DNA, and rapidly purify the PCR amplified products. The methods described are completed using the GenElute™ Blood Genomic DNA Kit, GenomePlex® WGA kit, and GenElute™ PCR Clean-up Kit.

 


The GenomePlex® WGA kit is the first whole genome amplification method to be applied to formalin-fixed paraffin-embedded (FFPE) tissues. Although archived FFPE tissues provide the convenience of storing samples for a long period of time, genomic DNA extracted from the samples can be damaged and of low yield. This can limit the researchers ability to perform downstream analysis. GenomePlex® WGA kit provides a method of amplifying nanogram quantities of FFPE extracted DNA with little or no detectable bias.

 


Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged and of low yield. These qualitites challenge the researchers ability to perform downstream analysis. GenomePlex® WGA provides a method of amplifying nanogram quantities of genomic DNA from plant with little or no detectable bias. The methods described use the GenElute™ Plant Genomic DNA Miniprep Kit.

 


This protocol addresses the need for extracting, amplifying, and purifying genomic DNA from soil samples. Genomic DNA from the soil samples can be damaged and of low yield. These qualities hurt the researchers ability to perform downstream analysis. GenomePlex® WGA provides a method of amplifying nanogram quantities of genomic DNA from soil with little or no detectable bias. The methods described use a soil extraction kit, GenomePlex® WGA kit, and GenElute™ PCR Clean-up Kit.

 


Back to Whole Genome Amplification