Whole Genome Amplification

Whole Transcriptome Amplification

Rapid Amplification of Total RNA

Amplify RNA, WTA2-TransPlex, Whole Transcriptome AmplificationThe TransPlex Whole Transcriptome Amplification (WTA1) and the Complete Whole Transcriptome Amplification Kit (WTA2) provide an accurate, fast and simple method of amplifying total RNA from a variety of sources including blood, fixed and frozen tissue, cell culture, FACS sorted cells, plants and microorganisms. The WTA technology accurately amplifies total RNA by the utilization of a unique blend of quasi-random primers to ensure accurate transcriptome coverage and rapid amplification of total RNA. The resulting cDNA is suitable for qPCR, micro array, and traditional cloning.

Product Description
The WTA kits provide rapid amplification of total RNA in less than 4 hours without 3’-bias. Amplified RNA (cDNA) is suitable for qPCR, micro array analysis, and traditional cloning. The WTA process has two steps; 1) library synthesis and 2) library amplification. To synthesize the cDNA library, sample RNA is incubated with reverse transcriptase and non-self-complementary primers comprised of a quasi-random 3’ end and a universal 5’ end. Annealed primers are extended by WTA polymerase, and displaced single strands become new templates for primer annealing and extension. The process creates an cDNA library comprised of random, overlapping 100-1000 base fragments, flanked by a universal end sequence. Finally, universal-primer PCR amplifies the cDNA library to produce microgram quantities of WTA products.

Product Number Product Description Reactions
WTA1-10RXN TransPlex Whole Transcriptome Amplification (WTA) Kit 10 reactions
WTA1-50RXN TransPlex Whole Transcriptome Amplification (WTA) Kit 50 reactions
WTA2-10RXN Complete Whole Transcriptome Amplification (WTA) Kit 10 reactions
WTA2-50RXN Complete Whole Transcriptome Amplification (WTA) Kit 50 reactions

NEW! Labeling Protocols for Microarrays

FAQs

TransPlex Whole Transcriptome Amplification Kit FAQs

Posters and Publications

Modification of the WTA2 Amplification Product for Next Generation Sequencing (1.61 Mb PDF)
Contact techserv@sial.com to participate as an early adopter of the WTA2-Sequencing technology.
Accurate Expression Profiling of Very Small Cell Populations (520 Kb PDF)
Virus-Plus-Susceptibility Gene Interaction Determines Crohn's Disease Gene Atg16L1 Phenotypes in Intestine (1.2 Mb PDF)
WTA2 on FFPE samples a comparison with NuGen (2.99 Mb PDF)
WTA2 amplification of very low RNA input and single cells, using qPCR analysis (1.25 Mb PDF)
Expression Profiling of Fixed and Unfixed Tissue Using Transplex™ Whole Transcriptome Amplification Kits (140 Kb PDF)

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