Whole Transcriptome Amplification

Rapid Amplification of Total RNA

The TransPlex Whole Transcriptome Amplification (WTA1 and WTA2) Kits provide an accurate, fast and simple method of amplifying total RNA from a variety of sources including blood, fixed and frozen tissue, cell culture, FACS sorted cells, plants and microorganisms. The TransPlex WTA technology accurately amplifies total RNA by the utilization of a unique blend of quasi-random primers to ensure accurate transcriptome coverage and rapid amplification of total RNA. The resulting cDNA is suitable for qPCR, micro array, and traditional cloning.

Product Description
The TransPlex WTA kits provide rapid amplification of total RNA in less than 4 hours without 3’-bias. Amplified RNA (cDNA) is suitable for qPCR, micro array analysis, and traditional cloning. The TransPlex WTA process has two steps; 1) library synthesis and 2) library amplification. To synthesize the cDNA library, sample RNA is incubated with reverse transcriptase and non-self-complementary primers comprised of a quasi-random 3’ end and a universal 5’ end. Annealed primers are extended by WTA polymerase, and displaced single strands become new templates for primer annealing and extension. The process creates an OmniPlex cDNA library comprised of random, overlapping 100-1000 base fragments, flanked by a universal end sequence. Finally, universal-primer PCR amplifies the OmniPlex^® cDNA library to produce microgram quantities of WTA products.

TransPlex Whole Transcriptome Amplification Kit FAQs

Expression Profiling of Fixed and Unfixed Tissue Using Transplex™
Whole Transcriptome Amplification Kits (140 Kb PDF)

Whole Transcriptome Amplification from Degraded RNA Transcripts (1.09 Mb PDF)

FACS Sorting of Stem Cells and Whole Transcriptome Amplification (111 Kb PDF)

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