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Proteomics

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ProteoPrep™ Plasma Immunodepletion | AQUA™ Peptides | Mass Spec Tools | Post-Translational Modification |
Panorama® Antibody Arrays | Recombinant Protein Expression & Purification | Cloning & Expression | Protein Detection


ProteoPrep™ Plasma Immunodepletion

Examination of Non-Specifically Bound Proteins and Peptides Following Depletion of Twenty High Abundance Proteins from Human Plasma (211 Kb)

Absolute Quantification of the Lower Abundance Proteome Through Immunoaffinity Depletion of the Twenty Most Abundant Proteins in Human Serum. (466 kb PDF)

High Specificity Depletion of Twenty High Abundance Proteins from Human Plasma Facilitates Examination of Plasma Proteome Differences Between Individuals. (270 kb PDF)

Specific Depletion of Twenty High Abundance Proteins from Human Plasma. (212 kb PDF)

Comparison Of Precipitation Methods Following Depletion Of Twenty High Abundance Proteins From Human Plasma. (204 kb PDF)

Novel Immunoaffinity Depletion Technology With Increased Binding Capacity Removes Approximately 97% of High Abundance Human Plasma Proteins (486 kb PDF)

Development of a Novel Antibody-Based Resin for the Depletion of Human Albumin and IgG (206 kb PDF)

Differing Isoform Pattern Changes to Human Plasma Proteins Produced by Covalently Modifying Protease Inhibitors and the Need for Protease Inhibitors (292 kb PDF)

The Protease Inhibitor 4-(2-Aminoethyl) Benzenesulfonyl Fluoride Covalently Modifies Human Plasma Proteins and Changes Protein Isoform Profiles (1.17 mb PDF)

Proteolytic Activity Quantitation and Protease Inhibition in Human Serum and Plasma Samples for Proteomic Analysis (388 kb PDF)

Identification Of Human Serum Proteins By 2DE And MALDI Mass Spectrometry Otherwise Masked By Albumin Using The ProteoPrep™ Blue Albumin Depletion Kit (164 kb PDF)


AQUA™ Peptides

Absolute Quantification (AQUA™) of Phosphorylation Levels in Biological Samples using Immobilized Metal Affinity Chromatography Enrichment (IMAC). (663 kb PDF)

Incorporation of an Internal Trypsin Cleavage Site into Protein-AQUA™ Standards for Absolute Quantification. (733 kb PDF)

Functional Validation of RNAi Knockdown at the Protein Level, via Sequential Quantitative Proteomics Techniques (378 kb PDF)

Absolute Quantification as an Effective Tool for Measuring Protein Expression in Targeted Gene Knockdown Experiments (126 kb PDF)


Mass Spec Tools

Protein Expression Analysis in Targeted Gene RNAi Knockdown Experiments Utilizing Isotopic Labeling with 18O Water and Protein-AQUA™ Peptides (921 kb PDF)

MALDI-TOF MS Analysis of Proteins by an Enhanced, Rapid Guanidination Procedure (106 kb PDF)

Mass Spectrometric Quantitation of Differentially Expressed Protein From Stable Isotopically Labeled CHO Cell Media (177 kb PDF)

Evaluation of Differential Protein Expression Through the Combination of RNAi and Isotopic Labeling Using 18O Water (202 kb PDF)

Preparation of CHO Samples for Evaluating Changes at the Proteomic Level (130 kb PDF)

Utility of the Enzymatic Labeling Method for Stable Isotope Incorporation into Protein Digests (139 kb PDF)

High Density Immobilized Trypsin in a Spin Column Format for Rapid Proteolysis and Increased Sequence Coverage (186 Kb)

The Effect of Denaturant Selection on Tryptic Digestion: Comparing Rapid Proteolysis Using Trypsin Spin Columns to Traditional Solution Digestion (267 kb PDF)


Post-Translational Modification

Specific Enrichment and Identification of Azide Modified Glycoproteins using the Staudinger Ligation (858 Kb)

A Novel Silica-Based Immobilized Metal Affinity Chromatography (IMAC) Technology for the Enrichment of Phosphopeptides. (708 kb PDF)

Targeting the Phosphotyrosine Proteome Using an Immobilized Metal Affinity Chromatography Spin Column and MALDI Matrix Optimization for Mass Spectrometric Enhancement (319 kb PDF)

Comparison of Two IMAC Formats for the Enrichment of Phosphopeptides for Analysis by Mass Spectrometry (965 kb PDF)

A Rapid and Improved Chemical Method for Deglycosylation of Glycoproteins (114 kb PDF)


Panorama® Antibody Arrays

Screening and Profiling Protein Expression in Human Cancer Serum using Antibody Array Technologies (267 Kb)

Panorama® Ab Microarray – Cell Signaling: A Valuable Tool for Studying the Differential Expression of Proteins in Cells (222 kb PDF)


Recombinant Protein Expression & Purification

A New TAP System for Isolation of Plant Protein Complexes and Subsequent Mass-Spec Analysis (528 kb PDF)

Binding & Kinetic Analysis of Protein Interactions Probed by Label–Free SpotMatrix SPR Technology in a Peptide Chip Format (287 kb PDF)

Capture of Tagged Proteins and Complexes with Enhanced Visibility Affinity Resins (137 kb PDF)

Improved Methods for Nuclear Extract Preparation and Functional Nuclei Isolation (93 kb PDF)

Rapid and Efficient Recovery of Histidine-Tagged Proteins Directly From Bacterial Cultures (407 kb PDF)

One Step Purification of HIS-tagged Fusion Proteins under High Pressure and High Flow Conditions (850 kb PDF)

Affinity-Purified ANTI-FLAG® M2 Antibody with High Specificity and Sensitivity (153 kb PDF)

Expression, Purification and Detection of Recombinant Fusion Proteins Using the MAT™ Tag System (383 kb PDF)

Affinity Capture Resins With Enhanced Visibility (80 kb PDF)

High Capacity Capture of Histidine-Tagged Recombinant Proteins (111 kb PDF)

High Throughput Platform Suitable for Common Proteomic Techniques (211 kb PDF)

Improved Affinity Resins for Immunoprecipitation (124 kb PDF)

Improved Methods for Isolation of Nuclei from Mammalian Cells (158 kb PDF)

The MAT™ Tag System*: A Novel Histidine–based Metal Affinity Tag (MAT) System for Expression, Purification and Detection of Recombinant Fusion Proteins (86 kb PDF)

Whole Bacterial Culture Lysis for Affinity Purification of Histidine-tagged Proteins (407 kb PDF)

Nitrilotriacetate-Atto Dye Conjugates For The Fluorescence Detection Of Oligo-Histidine Tagged Proteins (520 kb PDF)


Cloning & Expression

Development of a Novel High Throughput Directional Cloning Platform (56 kb PDF)

Development of Heterogeneous and Homogeneous Platforms for Rapid Analysis of DNA-Protein Interactions (142 kb PDF)

S-Gal™: An Autoclavable, Water-Soluble Dye for Enhanced Color-Selection of Cloned DNA Inserts (85 kb PDF)

Exonuclease III-Based High Throughput Construction of DNA Templates for In Vitro Expression (80 kb PDF)


Protein Detection

New Fluorescence Dyes For Protein Gel Stains (217 kb PDF)




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