Mass Spectrometry

MS PhosphoMix Standards

The MS PhosphoMix line of products allows for the testing of the strengths and weaknesses of phosphopeptide enrichment techniques, sample processing, and mass spectrometry based phosphoproteomics workflows. The products are also ideal reference materials to be used as “spike-ins” to gauge overall platform performance (e.g. recovery, sensitivity, repeatability) during routine phosphopeptide analysis.

The MS PhosphoMix consists of mono-, di-, tri-, and tetra-phosphorylated synthetic peptides formulated into 3 uniquely designed mixtures (MS PhosphoMix 1, 2 and 3, see below). Each mixture is available in natural (light) and isotopically labeled (heavy) versions making the set of products highly amenable to quantitative analyses, allowing users to compare recovery between workflows or enrichment techniques. The complete set of 25 phosphopeptide sequences, containing a total of 46 phosphorylation sites on serine, threonine, and tyrosine residues are derived from human proteins, as identified by Mann et al. in HeLa cells.1 Many natural phosphorylation motifs, such as those that present an abundance of proline, are represented.2

Each of the three mixtures were designed to include phosphopeptides having a broad range of characteristics including peptide ionizability (both ESI and MALDI), molecular weight, chromatographic retention time, charge state, and isoelectric point. An additional design consideration and product highlight is the inclusion of complimentary peptides that have the same or similar amino acid sequence but in which the number of phosphorylated residues and/or site-specific location of phosphorylation vary.

  • Mono-, di-, tri- and tetraphosphorylated peptides
  • 25 total sequences, 46 total sites of phosphorylation
  • Naturally occurring peptide sequences and phosphorylation motifs
  • Broad range of peptide characteristics
  • Complementary product designs
  • Available in light and heavy versions

 


MS PhosphoMix Product Concept

 

Mix 1 and 2 (10 peptides each)
  • Singly and doubly phosphorylated peptides

Mix 3 Contains (5 peptides)
  • Tri and tetra phosphorylated peptides
} Light and Heavy (Isotopically labeled) version of each



 


Ordering Information

 

Description Product No. Amount per Vial Amount per Peptide Add to Cart
MS PhosphoMix 1 Light MSP1L-1VL 200 pmol 20 pmol/peptide
MS PhosphoMix 1 Heavy MSP1H-1VL 200 pmol 20 pmol/peptide
MS PhosphoMix 2 Light MSP2L-1VL 200 pmol 20 pmol/peptide
MS PhosphoMix 2 Heavy MSP2H-1VL 200 pmol 20 pmol/peptide
MS PhosphoMix 3 Light MSP3L-1VL 200 pmol 40 pmol/peptide
MS PhosphoMix 3 Heavy MSP3H-1VL 200 pmol 40 pmol/peptide

 


Composition of PhosphoMix Products


Composition of PhosphoMix ProductsComposition of PhosphoMix Products

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ChromatogramsChromatograms

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Complementary Peptide Series

Complementary Peptide SeriesComplementary Peptide Series

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Complementary Peptide SeriesComplementary Peptide Series

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References

  1. Olsen, J. V. et al. Cell, 2006, 127: 635-648.
  2. Schwatz, D. et al. Nat. Biotech., 2005 23(11): 1391-1398.

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