UPS1 & UPS2 Proteomic Standards

Sigma^® now offers both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry. Both standards contain the same 48 human proteins ranging in molecular mass from 6,000 to 83,000 Daltons. Each constituent protein has been HPLC purified and AAA quantitated prior to formulation.

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UPS1 | UPS2 | References | 2006 ABRF Study | List of UPS Proteins

Download the new UPS FASTA File (17 Kb)

Universal Proteomics Standard, UPS1

Developed in collaboration with the Association of Biomolecular Resource Facilities Proteomics Standards Research Group (sPRG), the Universal Proteomics Standard (UPS1) contains 48 human proteins (5 pmols of each) ranging in molecular weight from 6,000 to 83,000 daltons.

NEW Proteomics Dynamic Range Standard, UPS2

This new standard is an enhancement of Sigma's original Universal Proteomics Standard (UPS1). The same complex mixture of 48 human proteins has been formulated into a dynamic range of concentrations, ranging from 500 amoles to 50 pmoles.

Ordering Information
Product No.	Product Name	Pkg Size	Add to Cart
UPS1	Universal Proteomics Standard	1 set
UPS2	Proteomics Dynamic Range Standard	1 set

Each set contains one vial of Universal Proteomics Standard and one vial (20 µg) of Proteomics Grade Trypsin (T6567).

References

Title	Author / Journal
Characterization of a Complex Protein Mixture Using nanoLC-LIT-TOF Mass Spectrometry.	M. Alexander Shaw, et al. Spectroscopy Mar 1, 2008
Methods, algorithms and tools in computational proteomics: a practical point of view..	Matthiesen R. Proteomics 2007 Aug. 7(16):2815-3223
Comparison of mascot and X!tandem performance for low and high accuracy mass spectrometry and the development of an adjusted mascot threshold.	Markus Brosch, et al. Mol Cell Proteomics 2008 Jan 23 [Epub ahead of print]
MyriMatch: Highly Accurate Tandem Mass Spectral Peptide Identification by Multivariate Hypergeometric Analysis.	Tabb, D. L.; et al. J. Proteome Res. 2007; 6(2), 654-661.
Computational approach for identification and characterization of GPI-anchored peptides in proteomics experiments .	Omaetxebarria, M. J.; et al. Proteomics 2007 June; 7(12), 1951-1960.
Interrogation of MS/MS search data with an pI Filter algorithm to increase protein identification success.	Nkemdilim C. Uwaje; et al. Electrophoresis 2007 May 22; 28(12), 1867-1874.
Unique Ion Signature Mass Spectrometry: a deterministic method to assign peptide identity.	Sherman, J., et al. Molecular & Cellular Proteomics 2009, June 25.
Comprehensive Comparison of Collision Induced Dissociation and Electron Transfer Dissociation.	Pandey, A., et al. Analytical Chemistry 2008; 80(13) 4825-4835.

The ABRF sPRG 2006 Study
In the Fall and Winter of 2005/2006, the ABRF sPRG (Proteomics Standards Research Group) conducted a study to assess the analytical capabilities of proteomics laboratories. Approximately 125 labs from across the world volunteered to participate. Each lab received a complex mixture of 49 unknown proteins and were asked to identify as many of these proteins as possible using their best analytical strategies. The results, presented in February 2006, were quite impressive and in some cases, surprising. Learn more about the sPRG’s 2006 study.

List of UPS Proteins

UniProt Accession Number	UPS1 Amount (fmol)	UPS2 Amount (fmol)	UniProt Protein Name [Synonym]	MW (Da) (calculated)	Source or recombinant	Host	Tag	Potential PTMs
P00915	5,000	50,000	Carbonic anhydrase 1	28,738	Erythrocytes			Acetylation
P00918	5,000	50,000	Carbonic anhydrase 2	29,095	Erythrocytes			Acetylation
P01031	5,000	50,000	Complement C5 [Complement C5a]	8,266	Recombinant	E. coli
P69905	5,000	50,000	Hemoglobin alpha chain	15,127	Erythrocytes
P68871	5,000	50,000	Hemoglobin beta chain	15,867	Erythrocytes			Acetylation, nitrosylation, glycosylation
P41159	5,000	50,000	Leptin	16,024	Recombinant	E. coli
P02768	5,000	50,000	Serum Albumin	66,393	Recombinant	Pichia pastoris
P62988	5,000	50,000	Ubiquitin	9,387	Recombinant	E. coli	6-His
P04040	5,000	50,000	Catalase	59,583	Erythrocytes
P00167	5,000	50,000	Cytochrome b₅	16,021	Recombinant	E. coli	6-His
P01133	5,000	50,000	Epidermal Growth Factor	6,211	Recombinant	E. coli
P02144	5,000	50,000	Myoglobin C	17,051	Heart
P15559	5,000	50,000	NAD(P)H dehydrogenase [quinone] 1 [DT Diaphorase] C	30,984	Recombinant	E. coli
P62937	5,000	50,000	Peptidyl-prolyl cis-trans isomerase A [Cyclophilin A]	17,947	Recombinant	E. coli
Q06830	5,000	50,000	Peroxiredoxin 1	22,106	Recombinant	E. coli
P63165	5,000	50,000	Small ubiquitin-related modifier 1 [SUMO-1]	37,420	Recombinant	E. coli	GST
P00709	5,000	500	Alpha-lactalbumin	14,070	Milk			Glycosylation
P06732	5,000	500	Creatine kinase M-type [CK-MM]	43,070	Heart
P12081	5,000	500	Histidyl-tRNA synthetase [Jo-1]	58,223	Recombinant	E. coli
P61626	5,000	500	Lysozyme C	14,692	Milk
Q15843	5,000	500	Neddylin [Nedd8]	9,071	Recombinant	E. coli
P02753	5,000	500	Retinol-binding protein	21,065	Urine
P16083	5,000	500	Ribosyldihydronicotinamide dehydrogenase [quinone] [Quinone oxidoreductase 2] [NQO2]	25,817	Recombinant	E. coli
P63279	5,000	500	Ubiquitin-conjugating enzyme E2 I [UbcH9]	22,907	Recombinant	E. coli
P01008	5,000	50	Antithrombin-III	49,033	Plasma			Glycosylation
P61769	5,000	50	Beta-2-microglobulin	11,729	Urine
P55957	5,000	50	BH3 Interacting domain death agonist [BID]	21,978	Recombinant	E. coli
P07339	5,000	50	Cathepsin D	26,624	Liver			Glycosylation
P08263	5,000	50	Glutathione S-transferase A1 [GST A1-1]	25,482	Recombinant	E. coli
P01344	5,000	50	Insulin-like growth factor II	7,464	Recombinant	E. coli
P01127	5,000	50	Platelet-derived growth factor B chain	12,286	Recombinant	E. coli
P10599	5,000	50	Thioredoxin	12,424	Recombinant	E. coli	6-His
P08311	5,000	5	Cathepsin G	26,751	Sputum			Glycosylation
P99999	5,000	5	Cytochrome c[Apocytochrome c]	11,608	Recombinant	E. coli
P06396	5,000	5	Gelsolin	82,954	Plasma			Phosphorylation
P09211	5,000	5	Glutathione S-transferase P [GST]	23,220	Placenta
P01112	5,000	5	GTPase HRas [Ras protein]	21,292	Recombinant	E. coli
P02787	5,000	5	Serotransferrin [Apotransferrin]	75,143	Plasma			Glycosylation
O00762	5,000	5	Ubiquitin-conjugating enzyme E2 C [UbcH10]	20,473	Recombinant	E. coli	6-His
P51965	5,000	5	Ubiquitin-conjugating enzyme E2 E1 [UbcH6]	22,222	Recombinant	E. coli	6-His
P08758	5,000	0.5	Annexin A 5	35,782	Placenta			Acetylation
P02741	5,000	0.5	C-reactive protein	23,030	Plasma
P05413	5,000	0.5	Fatty acid-binding protein	14,716	Plasma			Acetylation, phosphorylation
P10145	5,000	0.5	Interleukin-8	8,381	Recombinant	E. coli
P02788	5,000	0.5	Lactotransferrin	78,289	Milk			Glycosylation
P10636	5,000	0.5	Microtubule-associated protein tau [Tau protein]	46,810	Recombinant	E. coli	6-His
P00441	5,000	0.5	Superoxide dismutase [Cu-Zn]	15,800	Erythrocytes			Acetylation
P01343	5,000	0.5	Insulin-like growth factor IA	7,643	Recombinant	E. coli
P01375	5,000	0.5	Tumor necrosis factor [TNF-alpha]	17,350	Recombinant	E. coli

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