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Protein Mass Spectrometry
UPS1 & UPS2 Proteomic Standards
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Sigma® now offers both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry. Both standards contain the same 48 human proteins ranging in molecular mass from 6,000 to 83,000 Daltons. Each constituent protein has been HPLC purified and AAA quantitated prior to formulation.
- Troubleshoot and optimize your analytical protocol
- Confirm system suitability before analyzing critical samples
- Normalize analytical results day-to-day or lab-to-lab
- Determine your limit of detection
UPS1 | UPS2 | References | 2006 ABRF Study | List of UPS Proteins
Universal Proteomics Standard, UPS1
Developed in collaboration with the Association of Biomolecular Resource Facilities Proteomics Standards Research Group (sPRG), the Universal Proteomics Standard (UPS1) contains 48 human proteins (5 pmols of each) ranging in molecular weight from 6,000 to 83,000 daltons.
Proteomics Dynamic Range Standard, UPS2
This standard is an enhancement of Sigma's original Universal Proteomics Standard (UPS1). The same complex mixture of 48 human proteins has been formulated into a dynamic range of concentrations, ranging from 500 amoles to 50 pmoles.
| Ordering Information |
| Product No. |
Product Name |
Package Size |
Add to Cart |
| UPS1 |
Universal Proteomics Standard |
1 set |
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| UPS2 |
Proteomics Dynamic Range Standard |
1 set |
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Each set contains one vial of Universal Proteomics Standard and one vial (20 µg) of Proteomics Grade Trypsin (T6567).
References
| Title |
Author / Journal |
| Characterization of a Complex Protein Mixture Using nanoLC-LIT-TOF Mass Spectrometry. |
M. Alexander Shaw, et al.
Spectroscopy Mar 1, 2008 |
| Methods, algorithms and tools in computational proteomics: a practical point of view. |
Matthiesen R.
Proteomics 2007 Aug. 7(16):2815-3223 |
| Comparison of mascot and X!tandem performance for low and high accuracy mass spectrometry and the development of an adjusted mascot threshold. |
Markus Brosch, et al.
Mol Cell Proteomics 2008 Jan 23 [Epub ahead of print] |
| MyriMatch: Highly Accurate Tandem Mass Spectral Peptide Identification by Multivariate Hypergeometric Analysis. |
Tabb, D. L.; et al.
J. Proteome Res. 2007; 6(2), 654-661. |
| Computational approach for identification and characterization of GPI-anchored peptides in proteomics experiments. |
Omaetxebarria, M. J.; et al.
Proteomics 2007 June; 7(12), 1951-1960. |
| Interrogation of MS/MS search data with an pI Filter algorithm to increase protein identification success. |
Nkemdilim C. Uwaje; et al.
Electrophoresis 2007 May 22; 28(12), 1867-1874. |
| Unique Ion Signature Mass Spectrometry: a deterministic method to assign peptide identity. |
Sherman, J., et al.
Molecular & Cellular Proteomics 2009, June 25. |
| Comprehensive Comparison of Collision Induced Dissociation and Electron Transfer Dissociation. |
Pandey, A., et al.
Analytical Chemistry 2008; 80(13) 4825-4835. |
The ABRF sPRG 2006 Study
In the Fall and Winter of 2005/2006, the ABRF sPRG (Proteomics Standards Research Group) conducted a study to assess the analytical capabilities of proteomics laboratories. Approximately 125 labs from across the world volunteered to participate. Each lab received a complex mixture of 49 unknown proteins and were asked to identify as many of these proteins as possible using their best analytical strategies. The results, presented in February 2006, were quite impressive and in some cases, surprising. Learn more about the sPRG’s 2006 study.
List of UPS Proteins
| UniProt Accession Number |
UPS1 Amount (fmol) |
UPS2 Amount (fmol) |
UniProt Protein Name [Synonym] |
MW (Da)
(calculated) |
Source or recombinant |
Host |
Tag |
Potential PTMs |
| P00915 |
5,000 |
50,000 |
Carbonic anhydrase 1 |
28,738 |
Erythrocytes |
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Acetylation |
| P00918 |
5,000 |
50,000 |
Carbonic anhydrase 2 |
29,095 |
Erythrocytes |
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Acetylation |
| P01031 |
5,000 |
50,000 |
Complement C5 [Complement C5a] |
8,266 |
Recombinant |
E. coli |
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| P69905 |
5,000 |
50,000 |
Hemoglobin alpha chain |
15,127 |
Erythrocytes |
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| P68871 |
5,000 |
50,000 |
Hemoglobin beta chain |
15,867 |
Erythrocytes |
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Acetylation, nitrosylation, glycosylation |
| P41159 |
5,000 |
50,000 |
Leptin |
16,024 |
Recombinant |
E. coli |
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| P02768 |
5,000 |
50,000 |
Serum Albumin |
66,393 |
Recombinant |
Pichia pastoris |
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| P62988 |
5,000 |
50,000 |
Ubiquitin |
9,387 |
Recombinant |
E. coli |
6-His |
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| P04040 |
5,000 |
5,000 |
Catalase |
59,583 |
Erythrocytes |
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| P00167 |
5,000 |
5,000 |
Cytochrome b5 |
16,021 |
Recombinant |
E. coli |
6-His |
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| P01133 |
5,000 |
5,000 |
Epidermal Growth Factor |
6,211 |
Recombinant |
E. coli |
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| P02144 |
5,000 |
5,000 |
Myoglobin C |
17,051 |
Heart |
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| P15559 |
5,000 |
5,000 |
NAD(P)H dehydrogenase [quinone] 1 [DT Diaphorase] C |
30,984 |
Recombinant |
E. coli |
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| P62937 |
5,000 |
5,000 |
Peptidyl-prolyl cis-trans isomerase A [Cyclophilin A] |
17,947 |
Recombinant |
E. coli |
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| Q06830 |
5,000 |
5,000 |
Peroxiredoxin 1 |
22,106 |
Recombinant |
E. coli |
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| P63165 |
5,000 |
5,000 |
Small ubiquitin-related modifier 1 [SUMO-1] |
37,420 |
Recombinant |
E. coli |
GST |
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| P00709 |
5,000 |
500 |
Alpha-lactalbumin |
14,070 |
Milk |
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Glycosylation |
| P06732 |
5,000 |
500 |
Creatine kinase M-type [CK-MM] |
43,070 |
Heart |
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| P12081 |
5,000 |
500 |
Histidyl-tRNA synthetase [Jo-1] |
58,223 |
Recombinant |
E. coli |
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| P61626 |
5,000 |
500 |
Lysozyme C |
14,692 |
Milk |
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| Q15843 |
5,000 |
500 |
Neddylin [Nedd8] |
9,071 |
Recombinant |
E. coli |
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| P02753 |
5,000 |
500 |
Retinol-binding protein |
21,065 |
Urine |
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| P16083 |
5,000 |
500 |
Ribosyldihydronicotinamide dehydrogenase [quinone] [Quinone oxidoreductase 2] [NQO2] |
25,817 |
Recombinant |
E. coli |
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| P63279 |
5,000 |
500 |
Ubiquitin-conjugating enzyme E2 I [UbcH9] |
22,907 |
Recombinant |
E. coli |
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| P01008 |
5,000 |
50 |
Antithrombin-III |
49,033 |
Plasma |
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Glycosylation |
| P61769 |
5,000 |
50 |
Beta-2-microglobulin |
11,729 |
Urine |
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| P55957 |
5,000 |
50 |
BH3 Interacting domain death agonist [BID] |
21,978 |
Recombinant |
E. coli |
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| P07339 |
5,000 |
50 |
Cathepsin D |
26,624 |
Liver |
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Glycosylation |
| P08263 |
5,000 |
50 |
Glutathione S-transferase A1 [GST A1-1] |
25,482 |
Recombinant |
E. coli |
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| P01344 |
5,000 |
50 |
Insulin-like growth factor II |
7,464 |
Recombinant |
E. coli |
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| P01127 |
5,000 |
50 |
Platelet-derived growth factor B chain |
12,286 |
Recombinant |
E. coli |
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| P10599 |
5,000 |
50 |
Thioredoxin |
12,424 |
Recombinant |
E. coli |
6-His |
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| P08311 |
5,000 |
5 |
Cathepsin G |
26,751 |
Sputum |
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Glycosylation |
| P99999 |
5,000 |
5 |
Cytochrome c[Apocytochrome c] |
11,608 |
Recombinant |
E. coli |
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| P06396 |
5,000 |
5 |
Gelsolin |
82,954 |
Plasma |
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Phosphorylation |
| P09211 |
5,000 |
5 |
Glutathione S-transferase P [GST] |
23,220 |
Placenta |
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| P01112 |
5,000 |
5 |
GTPase HRas [Ras protein] |
21,292 |
Recombinant |
E. coli |
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| P02787 |
5,000 |
5 |
Serotransferrin [Apotransferrin] |
75,143 |
Plasma |
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Glycosylation |
| O00762 |
5,000 |
5 |
Ubiquitin-conjugating enzyme E2 C [UbcH10] |
20,473 |
Recombinant |
E. coli |
6-His |
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| P51965 |
5,000 |
5 |
Ubiquitin-conjugating enzyme E2 E1 [UbcH6] |
22,222 |
Recombinant |
E. coli |
6-His |
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| P08758 |
5,000 |
0.5 |
Annexin A 5 |
35,782 |
Placenta |
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Acetylation |
| P02741 |
5,000 |
0.5 |
C-reactive protein |
23,030 |
Plasma |
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| P05413 |
5,000 |
0.5 |
Fatty acid-binding protein |
14,716 |
Plasma |
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Acetylation, phosphorylation |
| P10145 |
5,000 |
0.5 |
Interleukin-8 |
8,381 |
Recombinant |
E. coli |
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| P02788 |
5,000 |
0.5 |
Lactotransferrin |
78,289 |
Milk |
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Glycosylation |
| P10636 |
5,000 |
0.5 |
Microtubule-associated protein tau [Tau protein] |
46,810 |
Recombinant |
E. coli |
6-His |
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| P00441 |
5,000 |
0.5 |
Superoxide dismutase [Cu-Zn] |
15,800 |
Erythrocytes |
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Acetylation |
| P01343 |
5,000 |
0.5 |
Insulin-like growth factor IA |
7,643 |
Recombinant |
E. coli |
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| P01375 |
5,000 |
0.5 |
Tumor necrosis factor [TNF-alpha] |
17,350 |
Recombinant |
E. coli |
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