Protein Mass Spectrometry

UPS1 & UPS2 Proteomic Standards

Sigma now offers both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry. Both standards contain the same 48 human proteins ranging in molecular mass from 6,000 to 83,000 Daltons. Each constituent protein has been HPLC purified and AAA quantitated prior to formulation.

  • Troubleshoot and optimize your analytical protocol
  • Confirm system suitability before analyzing critical samples
  • Normalize analytical results day-to-day or lab-to-lab
  • Determine your limit of detection

UPS1 | UPS2 | References | 2006 ABRF Study | List of UPS Proteins

 

Download the UPS FASTA File (17 Kb)


Universal Proteomics Standard, UPS1

Developed in collaboration with the Association of Biomolecular Resource Facilities Proteomics Standards Research Group (sPRG), the Universal Proteomics Standard (UPS1) contains 48 human proteins (5 pmols of each) ranging in molecular weight from 6,000 to 83,000 daltons.


Proteomics Dynamic Range Standard, UPS2

This standard is an enhancement of Sigma's original Universal Proteomics Standard (UPS1). The same complex mixture of 48 human proteins has been formulated into a dynamic range of concentrations, ranging from 500 amoles to 50 pmoles.

 

Ordering Information
Product No. Product Name Package Size Add to Cart
UPS1 Universal Proteomics Standard 1 set
UPS2 Proteomics Dynamic Range Standard 1 set

Each set contains one vial of Universal Proteomics Standard and one vial (20 µg) of Proteomics Grade Trypsin (T6567).


References

Title Author / Journal
Characterization of a Complex Protein Mixture Using nanoLC-LIT-TOF Mass Spectrometry. M. Alexander Shaw, et al.
Spectroscopy Mar 1, 2008
Methods, algorithms and tools in computational proteomics: a practical point of view. Matthiesen R.
Proteomics 2007 Aug. 7(16):2815-3223
Comparison of mascot and X!tandem performance for low and high accuracy mass spectrometry and the development of an adjusted mascot threshold. Markus Brosch, et al.
Mol Cell Proteomics 2008 Jan 23 [Epub ahead of print]
MyriMatch: Highly Accurate Tandem Mass Spectral Peptide Identification by Multivariate Hypergeometric Analysis. Tabb, D. L.; et al.
J. Proteome Res. 2007; 6(2), 654-661.
Computational approach for identification and characterization of GPI-anchored peptides in proteomics experiments. Omaetxebarria, M. J.; et al.
Proteomics 2007 June; 7(12), 1951-1960.
Interrogation of MS/MS search data with an pI Filter algorithm to increase protein identification success. Nkemdilim C. Uwaje; et al.
Electrophoresis 2007 May 22; 28(12), 1867-1874.
Unique Ion Signature Mass Spectrometry: a deterministic method to assign peptide identity. Sherman, J., et al.
Molecular & Cellular Proteomics 2009, June 25.
Comprehensive Comparison of Collision Induced Dissociation and Electron Transfer Dissociation. Pandey, A., et al.
Analytical Chemistry 2008; 80(13) 4825-4835.


The ABRF sPRG 2006 Study

In the Fall and Winter of 2005/2006, the ABRF sPRG (Proteomics Standards Research Group) conducted a study to assess the analytical capabilities of proteomics laboratories. Approximately 125 labs from across the world volunteered to participate. Each lab received a complex mixture of 49 unknown proteins and were asked to identify as many of these proteins as possible using their best analytical strategies. The results, presented in February 2006, were quite impressive and in some cases, surprising. Learn more about the sPRG’s 2006 study.


List of UPS Proteins

UniProt Accession Number UPS1 Amount (fmol) UPS2 Amount (fmol) UniProt Protein Name [Synonym] MW (Da)
(calculated)		 Source or recombinant Host Tag Potential PTMs
P00915 5,000 50,000 Carbonic anhydrase 1 28,738 Erythrocytes     Acetylation
P00918 5,000 50,000 Carbonic anhydrase 2 29,115 Erythrocytes     Acetylation
P01031 5,000 50,000 Complement C5 [Complement C5a] 8,536 Recombinant E. coli    
P69905 5,000 50,000 Hemoglobin alpha chain 15,126 Erythrocytes      
P68871 5,000 50,000 Hemoglobin beta chain 15,867 Erythrocytes     Acetylation, nitrosylation, glycosylation
P41159 5,000 50,000 Leptin 16,158 Recombinant E. coli    
P02768 5,000 50,000 Serum Albumin 66,357 Recombinant Pichia pastoris    
P62988 5,000 50,000 Ubiquitin 10,597 Recombinant E. coli 6-His  
P04040 5,000 5,000 Catalase 59,625 Erythrocytes      
P00167 5,000 5,000 Cytochrome b5 16,022 Recombinant E. coli 6-His  
P01133 5,000 5,000 Epidermal Growth Factor 6,353 Recombinant E. coli    
P02144 5,000 5,000 Myoglobin C 17,053 Heart      
P15559 5,000 5,000 NAD(P)H dehydrogenase [quinone] 1 [DT Diaphorase] C 30,736 Recombinant E. coli    
P62937 5,000 5,000 Peptidyl-prolyl cis-trans isomerase A [Cyclophilin A] 20,176 Recombinant E. coli    
Q06830 5,000 5,000 Peroxiredoxin 1 21,979 Recombinant E. coli    
P63165 5,000 5,000 Small ubiquitin-related modifier 1 [SUMO-1] 38,815 Recombinant E. coli GST  
P00709 5,000 500 Alpha-lactalbumin 14,078 Milk     Glycosylation
P06732 5,000 500 Creatine kinase M-type [CK-MM] 43,101 Heart      
P12081 5,000 500 Histidyl-tRNA synthetase [Jo-1] 58,233 Recombinant E. coli    
P61626 5,000 500 Lysozyme C 14,701 Milk      
Q15843 5,000 500 Neddylin [Nedd8] 9,072 Recombinant E. coli    
P02753 5,000 500 Retinol-binding protein 21,071 Urine      
P16083 5,000 500 Ribosyldihydronicotinamide dehydrogenase [quinone] [Quinone oxidoreductase 2] [NQO2] 25,821 Recombinant E. coli    
P63279 5,000 500 Ubiquitin-conjugating enzyme E2 I [UbcH9] 18,007 Recombinant E. coli    
P01008 5,000 50 Antithrombin-III 49,039 Plasma     Glycosylation
P61769 5,000 50 Beta-2-microglobulin 11,731 Urine      
P55957 5,000 50 BH3 Interacting domain death agonist [BID] 21,995 Recombinant E. coli    
O76070 5,000 50 Gamma-synuclein 15,363 Recombinant E. coli    
P08263 5,000 50 Glutathione S-transferase A1 [GST A1-1] 25,500 Recombinant E. coli    
P01344 5,000 50 Insulin-like growth factor II 7,475 Recombinant E. coli    
P01127 5,000 50 Platelet-derived growth factor B chain 12,294 Recombinant E. coli    
P10599 5,000 50 Thioredoxin 12,429 Recombinant E. coli 6-His  
P99999 5,000 5 Cytochrome c[Apocytochrome c] 11,618 Recombinant E. coli    
P06396 5,000 5 Gelsolin 82,959 Plasma     Phosphorylation
P09211 5,000 5 Glutathione S-transferase P [GST] 23,225 Placenta      
P01112 5,000 5 GTPase HRas [Ras protein] 21,298 Recombinant E. coli    
P01579 5,000 5 Interferon gamma (IFN-gamma) 16,879 Recombinant E. coli    
P02787 5,000 5 Serotransferrin [Apotransferrin] 75,181 Plasma     Glycosylation
O00762 5,000 5 Ubiquitin-conjugating enzyme E2 C [UbcH10] 20,475 Recombinant E. coli 6-His  
P51965 5,000 5 Ubiquitin-conjugating enzyme E2 E1 [UbcH6] 22,227 Recombinant E. coli 6-His  
P08758 5,000 0.5 Annexin A 5 35,806 Placenta     Acetylation
P02741 5,000 0.5 C-reactive protein 23,047 Plasma      
P05413 5,000 0.5 Fatty acid-binding protein 14,727 Plasma     Acetylation, phosphorylation
P10145 5,000 0.5 Interleukin-8 8,386 Recombinant E. coli    
P02788 5,000 0.5 Lactotransferrin 76,165 Milk     Glycosylation
P10636 5,000 0.5 Microtubule-associated protein tau [Tau protein] 45,719 Recombinant E. coli 6-His  
P00441 5,000 0.5 Superoxide dismutase [Cu-Zn] 15,805 Erythrocytes     Acetylation
P01343 5,000 0.5 Insulin-like growth factor IA 7,643 Recombinant E. coli    
P01375 5,000 0.5 Tumor necrosis factor [TNF-alpha] 17,353 Recombinant E. coli    

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