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GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components. Reaction conditions may require optimization of denaturation time, reaction time, and PNGase F concentration for deglycosylation of different glycoproteins.
GlycoProfile II contains sufficient enzyme, glycoprotein standard, and reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.
Components:
Proteomics Grade PNGase F
Ribonuclease B (RNase B)-glycoprotein standard
10X Reaction Buffer
Octyl b-D-glucopyranoside
2-Mercaptoethanol - 0.90 ml (Product Code M 6250)
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