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Phosphorylation

Additional Products




PhosDecor™ Fluorescent Phosphoprotein In-Gel Detection Kit (PDECOR)

PhosDecor™ Fluorescent Phosphoprotein In-Gel Detection Kit provides quick and selective identification of phosphorylated proteins directly in SDS-polyacrylamide gels. This technology is particularly useful in screening for phosphoproteins in complex protein samples.

  • Complete in as little as 4 hours—provides quick identification of protein phosphorylation
  • Gels may be dual stained for total protein—measure variations in gel loading and identify non-phosphorylated proteins
  • Compatible with downstream MALDI-MS analysis—allows identification of specific phosphorylation sites
  • Linear detection of phosphoproteins over a wide range—allows for qualitative comparisons
  • A complete solution kit—saves time and reduces waste

The kit includes sufficient reagents for staining five mini-gels (8 cm × 8 cm × 1 mm).

PhosDecor Stain 1 × 250 ml
Fixing Solution 6 × 250 ml
Destaining Solution 2 × 250 ml
PhosDecor Control 1 vial


PhosDecor Stain Graph

Figure 1: Staining of β-casein ranging from 3.13 ng to 200 ng using a UV transilluminator.

PhosDecor allows for qualitative comparisons in phosphoproteins with comparable phosphorylation states due to a uniform signal response to the presence of phospho groups present.

Following PhosDecor staining, protein spots or bands can be digested using the Trypsin Profile IGD Kit (Catalog Number PP0100) and prepared for downstream MALDI-MS analysis. For accurate detection of phosphorylated peptides, digested proteins may need to be enriched prior to mass spectrometry (i.e., PhosphoProfile™ I Phosphopeptide Enrichment Kit, Catalog Number PP0410).

PhosDecor Mass Spectometry Graph

Figure 2: MALDI-MS spectra of ovalbumin stained with PhosDecor Stain.




Phosphopeptide Positive Control Set, (P9615)

This set contains mono-and tetra-phosphopeptide standards prepared by tryptic digestion of ß-casein. It is designed for use in phosphopeptide purification and characterization.




Monoclonal Antibodies and Other Products for Phosphoprotein Analysis

Sigma has developed antibodies directed against phosphotyrosine, phosphoserine and phosphothreonine residues. These antibodies are offered unconjugated and conjugated to biotin, agarose and other molecules. These are useful as analytical and preparative tools, enabling the identification, quantification and immunoaffinity isolation of phosphorylated proteins and peptides. Additional products for capture and detection are also listed.

  Antibodies for the Isolation of Phosphospeptides
Antibodies for Dectection of Phosphospeptides
Controls and Elution Reagents
Secondary Antibodies and Labeled Streptavidin
Western Blotting Kits
Substrates for Immunoblotting
Substrates for ELISA
Reagents and Buffers Immunoassays
Resins and Plates
Tyrosine Kinase Inhibitors
Phosphatase Detection Kit
Reagents and Standards
Serine/Threonine Kinase Inhibitors
Phosphatase Inhibitors

Antibodies for the Isolation of Phosphopeptides  
Product Name Product # 
Monoclonal Anti-Phosphotyrosine-Agarose from mouse, Purified immunoglobulin, PBS suspension, clone PT-66 A1806
Monoclonal Anti-Phosphoserine-Agarose, Purified immunoglobulin, PBS suspension, clone PSR-45 A8076
Monoclonal Anti-Phosphothreonine-Agarose from mouse, Purified immunoglobulin, PBS suspension A7951
Antibodies for Detection of Phosphopeptides back to top
Product Name Product # 
Monoclonal Anti-Phosphotyrosine, Unconjugated from mouse, Ascites fluid, Liquid, clone PT-66 P3300
Monoclonal Anti-Phosphotyrosine-Peroxidase Conjugate from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 A4595
Monoclonal Anti-Phosphotyrosine Peroxidase- Conjugate from mouse, Purified immunoglobulin, Lyophilized powder, clone PT-66 A5964
Monoclonal Anti-Phosphotyrosine- Biotin Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution, clone PT-66 B1531
Monoclonal Anti-Phosphotyrosine- Biotin Conjugate from mouse, IgG fraction of antiserum, Buffered aqueous glycerol solution, clone PY20 B5055
Monoclonal Anti-Phosphotyrosine- FITC Conjugate from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 F0426
Monoclonal Anti-Phosphotyrosine- FITC Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution, clone PT-66 F3145
Monoclonal Anti-Phosphotyrosine, Unconjugated from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 P4110
Monoclonal Anti-Phosphoserine, Unconjugated from mouse, Ascites fluid, Liquid, clone PSR-45 P3430
Monoclonal Anti-Phosphoserine-Biotin Conjugate, Purified immunoglobulin, Buffered aqueous solution B7911
Monoclonal Anti-Phosphoserine-FITC Conjugate, Purified immunoglobulin, Buffered aqueous solution F3662
Monoclonal Anti-Phosphothreonine, Unconjugated from mouse, Ascites fluid, Liquid, clone PTR-8 P3555
Monoclonal Anti-Phosphothreonine-Biotin Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution B7661
Monoclonal Anti-Phosphothreonine FITC Conjugate mouse, Purified immunoglobulin F3787
Controls and Elution Reagents back to top
Product Name Product # 
Phosphotyrosine-BSA Phosphotyrosine Antibody Inhibitor, 2 mg per ml, Buffered aqueous solution, for blotting and ELISA P3967
Phosphoserine-BSA, Phosphoserine Antibody Inhibitor, 2 mg/ml, for blotting and ELISA P3717
Phosphothreonine-BSA, Phosphothreonine Antibody Inhibitor, 2 mg/ml, for blotting and ELISA P3842
Sodium phenyl phosphate dibasic dihydrate, 95%, elutes phosphoproteins from antibody resins P7751
Secondary Antibodies and Labeled Streptavidin back to top
Product Name Product # 
Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase from goat, Affinity isolated antibody, Buffered aqueous glycerol solution A3562
Anti-Mouse IgG (whole molecule) Peroxidase from rabbit, IgG fraction of antiserum, Buffered aqueous solution A9044
Anti-Mouse IgG (Fc specific)–Biotin from goat, Affinity isolated antibody, Buffered aqueous solution B7401
Anti-Mouse IgG (whole molecule)–FITC from goat, Affinity isolated antibody, Buffered aqueous solution F0257
Streptavidin-Alkaline Phosphatase conjugate from Streptomyces avidinii, Lyophilized powder, 700-1,400 DEA units/mg protein S2890
Streptavidin-Peroxidase conjugate from Streptomyces avidinii, Lyophilized powder, 80-150 units/mg protein S5512
Western Blotting Kits back to top
Product Name Product # 
ProteoQwestä Chemiluminescent Western Blotting Kit, CPS Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0201
ProteoQwestä Colorimetric Western Blotting Kit, TMB Substrate, (for Mouse Monoclonal IgG Antibodies) PQ0101
Chemichromeä Western Control, (Color Molecular Weight Marker) C4236
Substrates for Immunoblotting back to top
Product Name Product # 
For Alkaline Phosphatase  
CDP-Star Chemiluminescent Substrate solution, 0.25 mM in water C0712
BCIP/NBT Liquid Substrate System B1911
SIGMA FASTä BCIP/NBT, Tablet B5655
Product Name Product # 
For Peroxidase  
Chemiluminescent peroxidase substrate CPS-1
SIGMA FASTä 3,3¢-Diaminobenzidine Tablets to make 15 ml D4418
SIGMA FASTä DAB with Metal Enhancer, Tablet, each makes 5 ml D0426
3,3¢,5,5¢-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, Liquid T0565
Substrates for ELISA back to top
Product Name Product # 
For Alkaline Phosphatase  
4-Nitrophenyl phosphate disodium salt solution, Liquid N7653
SIGMA FASTä p-Nitrophenyl Phosphate Tablets, Tablet, each makes 20 ml N2770
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, Liquid M3168
Product Name Product # 
For Peroxidase  
3,3¢,5,5¢-Tetramethylbenzidine (TMB) Liquid Substrate System, Liquid T8665
SIGMA FASTä OPD, Tablet P9187
Reagents and Buffers Immunoassays back to top
Product Name Product # 
Western Blocker Solution, for HRP detection systems W0138
Chemichromeä Western Control, (Color Molecular Weight Marker) C4236
Phosphate Buffered Saline with 5% nonfat milk, pH 7.3 from bovine milk, pH 7.3, Powder (dry milled) P4739
Phosphate Buffered Saline, pH 7.4, with 3% Nonfat Milk, Dry powder P2194
Phosphate Buffered Saline, pH 7.4, Powder, each pouch makes 1 L P3813
Phosphate Buffered Saline, pH 7.4, Tablet, each tablet makes 200 ml P4417
Phosphate-Citrate Buffer with Sodium Perborate, Capsule P4922
Carbonate-Bicarbonate Buffer, Capsule C3041
Tris buffered saline, pH 8.0, Powder, each pouch makes 1 L T6664
Tris buffered saline, pH 8.0, Tablet, each tablet makes 15 ml T5030
Resins and Plates back to top
Product Name Product # 
Avidin-agarose from egg white, Aqueous glycerol suspension A9207
Avidin (Monomeric)-Agarose from egg white, Aqueous glycerol suspension A2036
Streptavidin-Agarose from Streptomyces avidinii, Buffered aqueous suspension S1638
SigmaScreen ExtrAvidin Coated Plates E3027
SigmaScreen Streptavidin 96-well High Capacity Coated Plates S6940
SigmaScreen Streptavidin 384-well High Capacity Coated Plates S8815
Anti-Mouse IgG (whole molecule)-Agarose from goat, IgG fraction of antiserum, Saline suspension A6531
EZviewä Red Protein A Affinity Gel P6486
EZviewä Red Protein G Affinity Gel E3403
Protein L-Agarose from Peptostreptococcus magnus, recombinant, expressed in Escherichia coli P3351
Protein A coated HS 96-well plates, flat-bottomed 96-well plates, clear polystyrene S1938
Protein G coated HS 96-well plates, flat-bottomed 96-well plates, clear polystyrene S2063
Tyrosine Kinase Inhibitors back to top
Product Name Product # 
4,5-Dianilinophthalimide, Solid D-210
Emodin, >95%, Solid E-171
Geldanamycin from Streptomyces hygroscopicus, minimum 98% (HPLC), Powder G3381
Lavendustin A, approx. 85% (TLC), Crystalline L2400
Tyrphostin AG 490, Solid T3434
Tyrphostin AG 1478, minimum 90% T4182
Phosphatase Inhibitors back to top
Product Name Product # 
Cantharidic acid C8088
Okadaic acid sodium salt from Prorocentrum concavum, minimum 95% (HPLC) O7760
Phenylarsine oxide, minimum 97%, Powder P3075
Phosphatase Inhibitor Cocktail 1, DMSO solution P2850
Phosphatase Inhibitor Cocktail 2, Aqueous solution P5726
Sodium fluoride solution, minimum 99% S1504
Phosphatase Detection Kit back to top
Product Name Product # 
Protein Tyrosine Phosphatase (PTP) Assay Kit, Non-Radioactive PTP-101
Reagents and Standards back to top
Product Name Product # 
Ammonium molybdate tetrahydrate, ACS Reagent, 81.0-83.0% as MoO3 A7302
Barium hydroxide octahydrate, minimum 98.0% B2507
a-Casein from bovine milk, minimum 70% as as-casein (electrophoresis), Lyophilized powder C6780
b-Casein from bovine milk, minimum 90% as b-casein (electrophoresis), Essentially salt-free, lyophilized powder C6905
Albumin from chicken egg white, Grade V, minimum 98% (agarose gel electrophoresis), Lyophilized powder, ovalbumin A5503
Phosvitin from egg yolk P1253
Iron(II) sulfate heptahydrate, minimum 99.0%, (Ferrous sulfate) F7002
Sodium phenyl phosphate dibasic dihydrate, 95%, (Phenylphosphate) P7751
Phosphorous Standard 661-9
Phosphopeptide positive control set P9615
Malachite Green hydrochloride M9636
Fiske-SubbaRow Reducer 661-8
Serine/Threonine Kinase Inhibitors back to top
Product Name Product # 
1-(5-Isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7) H-121
ML-7, Powder I2764
PD 98,059, Solid P-215
SB 203770, Powder S8307
D-Sphingosine, bovine brain, approx. 99% (TLC) S6879

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