PhosDecor™ Fluorescent Phosphoprotein In-Gel Detection Kit (PDECOR)
PhosDecor™ Fluorescent Phosphoprotein In-Gel Detection Kit provides quick and selective identification of phosphorylated proteins directly in SDS-polyacrylamide gels. This technology is particularly useful in screening for phosphoproteins in complex protein samples.
- Complete in as little as 4 hours—provides quick identification of protein phosphorylation
- Gels may be dual stained for total protein—measure variations in gel loading and identify non-phosphorylated proteins
- Compatible with downstream MALDI-MS analysis—allows identification of specific phosphorylation sites
- Linear detection of phosphoproteins over a wide range—allows for qualitative comparisons
- A complete solution kit—saves time and reduces waste
The kit includes sufficient reagents for staining five mini-gels (8 cm × 8 cm × 1 mm).
PhosDecor Stain 1 × 250 ml
Fixing Solution 6 × 250 ml
Destaining Solution 2 × 250 ml
PhosDecor Control 1 vial
Figure 1: Staining of β-casein ranging from 3.13 ng to 200 ng using a UV transilluminator.
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PhosDecor allows for qualitative comparisons in phosphoproteins with comparable phosphorylation states due to a uniform signal response to the presence of phospho groups present. |
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Following PhosDecor staining, protein spots or bands can be digested using the Trypsin Profile IGD Kit (Catalog Number PP0100) and prepared for downstream MALDI-MS analysis. For accurate detection of phosphorylated peptides, digested proteins may need to be enriched prior to mass spectrometry (i.e., PhosphoProfile™ I Phosphopeptide Enrichment Kit, Catalog Number PP0410).
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Figure 2: MALDI-MS spectra of ovalbumin stained with PhosDecor Stain.
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Phosphopeptide Positive Control Set, (P9615)
This set contains mono-and tetra-phosphopeptide standards prepared by tryptic digestion of ß-casein. It is designed for use in phosphopeptide purification and characterization.
Monoclonal Antibodies and Other Products for Phosphoprotein Analysis
Sigma has developed antibodies directed against phosphotyrosine, phosphoserine and phosphothreonine residues. These antibodies are offered unconjugated and conjugated to biotin, agarose and other molecules. These are useful as analytical and preparative tools, enabling the identification, quantification and immunoaffinity isolation of phosphorylated proteins and peptides. Additional products for capture and detection are also listed.
| Antibodies for the Isolation of Phosphopeptides |
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| Product Name |
Product # |
| Monoclonal Anti-Phosphotyrosine-Agarose from mouse, Purified immunoglobulin, PBS suspension, clone PT-66 |
A1806 |
| Monoclonal Anti-Phosphoserine-Agarose, Purified immunoglobulin, PBS suspension, clone PSR-45 |
A8076 |
| Monoclonal Anti-Phosphothreonine-Agarose from mouse, Purified immunoglobulin, PBS suspension |
A7951 |
| Antibodies for Detection of Phosphopeptides |
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| Product Name |
Product # |
| Monoclonal Anti-Phosphotyrosine, Unconjugated from mouse, Ascites fluid, Liquid, clone PT-66 |
P3300 |
| Monoclonal Anti-Phosphotyrosine-Peroxidase Conjugate from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 |
A4595 |
| Monoclonal Anti-Phosphotyrosine Peroxidase- Conjugate from mouse, Purified immunoglobulin, Lyophilized powder, clone PT-66 |
A5964 |
| Monoclonal Anti-Phosphotyrosine- Biotin Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution, clone PT-66 |
B1531 |
| Monoclonal Anti-Phosphotyrosine- Biotin Conjugate from mouse, IgG fraction of antiserum, Buffered aqueous glycerol solution, clone PY20 |
B5055 |
| Monoclonal Anti-Phosphotyrosine- FITC Conjugate from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 |
F0426 |
| Monoclonal Anti-Phosphotyrosine- FITC Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution, clone PT-66 |
F3145 |
| Monoclonal Anti-Phosphotyrosine, Unconjugated from mouse, Purified immunoglobulin, Buffered aqueous glycerol solution, clone PY20 |
P4110 |
| Monoclonal Anti-Phosphoserine, Unconjugated from mouse, Ascites fluid, Liquid, clone PSR-45 |
P3430 |
| Monoclonal Anti-Phosphoserine-Biotin Conjugate, Purified immunoglobulin, Buffered aqueous solution |
B7911 |
| Monoclonal Anti-Phosphoserine-FITC Conjugate, Purified immunoglobulin, Buffered aqueous solution |
F3662 |
| Monoclonal Anti-Phosphothreonine, Unconjugated from mouse, Ascites fluid, Liquid, clone PTR-8 |
P3555 |
| Monoclonal Anti-Phosphothreonine-Biotin Conjugate from mouse, Purified immunoglobulin, Buffered aqueous solution |
B7661 |
| Monoclonal Anti-Phosphothreonine FITC Conjugate mouse, Purified immunoglobulin |
F3787 |
| Controls and Elution Reagents |
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| Product Name |
Product # |
| Phosphotyrosine-BSA Phosphotyrosine Antibody Inhibitor, 2 mg per ml, Buffered aqueous solution, for blotting and ELISA |
P3967 |
| Phosphoserine-BSA, Phosphoserine Antibody Inhibitor, 2 mg/ml, for blotting and ELISA |
P3717 |
| Phosphothreonine-BSA, Phosphothreonine Antibody Inhibitor, 2 mg/ml, for blotting and ELISA |
P3842 |
| Sodium phenyl phosphate dibasic dihydrate, 95%, elutes phosphoproteins from antibody resins |
P7751 |
| Secondary Antibodies and Labeled Streptavidin |
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| Product Name |
Product # |
| Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase from goat, Affinity isolated antibody, Buffered aqueous glycerol solution |
A3562 |
| Anti-Mouse IgG (whole molecule) Peroxidase from rabbit, IgG fraction of antiserum, Buffered aqueous solution |
A9044 |
| Anti-Mouse IgG (Fc specific)–Biotin from goat, Affinity isolated antibody, Buffered aqueous solution |
B7401 |
| Anti-Mouse IgG (whole molecule)–FITC from goat, Affinity isolated antibody, Buffered aqueous solution |
F0257 |
| Streptavidin-Alkaline Phosphatase conjugate from Streptomyces avidinii, Lyophilized powder, 700-1,400 DEA units/mg protein |
S2890 |
| Streptavidin-Peroxidase conjugate from Streptomyces avidinii, Lyophilized powder, 80-150 units/mg protein |
S5512 |
| Western Blotting Kits |
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| Product Name |
Product # |
| ProteoQwestä Chemiluminescent Western Blotting Kit, CPS Substrate, (for Mouse Monoclonal IgG Antibodies) |
PQ0201 |
| ProteoQwestä Colorimetric Western Blotting Kit, TMB Substrate, (for Mouse Monoclonal IgG Antibodies) |
PQ0101 |
| Chemichromeä Western Control, (Color Molecular Weight Marker) |
C4236 |
| Substrates for Immunoblotting |
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| Product Name |
Product # |
| For Alkaline Phosphatase |
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| CDP-Star Chemiluminescent Substrate solution, 0.25 mM in water |
C0712 |
| BCIP/NBT Liquid Substrate System |
B1911 |
| SIGMA FASTä BCIP/NBT, Tablet |
B5655 |
| Product Name |
Product # |
| For Peroxidase |
|
| Chemiluminescent peroxidase substrate |
CPS-1 |
| SIGMA FASTä 3,3¢-Diaminobenzidine Tablets to make 15 ml |
D4418 |
| SIGMA FASTä DAB with Metal Enhancer, Tablet, each makes 5 ml |
D0426 |
| 3,3¢,5,5¢-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, Liquid |
T0565 |
| Substrates for ELISA |
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| Product Name |
Product # |
| For Alkaline Phosphatase |
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| 4-Nitrophenyl phosphate disodium salt solution, Liquid |
N7653 |
| SIGMA FASTä p-Nitrophenyl Phosphate Tablets, Tablet, each makes 20 ml |
N2770 |
| 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, Liquid |
M3168 |
| Product Name |
Product # |
| For Peroxidase |
|
| 3,3¢,5,5¢-Tetramethylbenzidine (TMB) Liquid Substrate System, Liquid |
T8665 |
| SIGMA FASTä OPD, Tablet |
P9187 |
| Reagents and Buffers Immunoassays |
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| Product Name |
Product # |
| Western Blocker Solution, for HRP detection systems |
W0138 |
| Chemichromeä Western Control, (Color Molecular Weight Marker) |
C4236 |
| Phosphate Buffered Saline with 5% nonfat milk, pH 7.3 from bovine milk, pH 7.3, Powder (dry milled) |
P4739 |
| Phosphate Buffered Saline, pH 7.4, with 3% Nonfat Milk, Dry powder |
P2194 |
| Phosphate Buffered Saline, pH 7.4, Powder, each pouch makes 1 L |
P3813 |
| Phosphate Buffered Saline, pH 7.4, Tablet, each tablet makes 200 ml |
P4417 |
| Phosphate-Citrate Buffer with Sodium Perborate, Capsule |
P4922 |
| Carbonate-Bicarbonate Buffer, Capsule |
C3041 |
| Tris buffered saline, pH 8.0, Powder, each pouch makes 1 L |
T6664 |
| Tris buffered saline, pH 8.0, Tablet, each tablet makes 15 ml |
T5030 |
| Resins and Plates |
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| Product Name |
Product # |
| Avidin-agarose from egg white, Aqueous glycerol suspension |
A9207 |
| Avidin (Monomeric)-Agarose from egg white, Aqueous glycerol suspension |
A2036 |
| Streptavidin-Agarose from Streptomyces avidinii, Buffered aqueous suspension |
S1638 |
| SigmaScreen ExtrAvidin Coated Plates |
E3027 |
| SigmaScreen Streptavidin 96-well High Capacity Coated Plates |
S6940 |
| SigmaScreen Streptavidin 384-well High Capacity Coated Plates |
S8815 |
| Anti-Mouse IgG (whole molecule)-Agarose from goat, IgG fraction of antiserum, Saline suspension |
A6531 |
| EZviewä Red Protein A Affinity Gel |
P6486 |
| EZviewä Red Protein G Affinity Gel |
E3403 |
| Protein L-Agarose from Peptostreptococcus magnus, recombinant, expressed in Escherichia coli |
P3351 |
| Protein A coated HS 96-well plates, flat-bottomed 96-well plates, clear polystyrene |
S1938 |
| Protein G coated HS 96-well plates, flat-bottomed 96-well plates, clear polystyrene |
S2063 |
| Tyrosine Kinase Inhibitors |
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| Product Name |
Product # |
| 4,5-Dianilinophthalimide, Solid |
D-210 |
| Emodin, >95%, Solid |
E-171 |
| Geldanamycin from Streptomyces hygroscopicus, minimum 98% (HPLC), Powder |
G3381 |
| Lavendustin A, approx. 85% (TLC), Crystalline |
L2400 |
| Tyrphostin AG 490, Solid |
T3434 |
| Tyrphostin AG 1478, minimum 90% |
T4182 |
| Phosphatase Inhibitors |
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| Product Name |
Product # |
| Cantharidic acid |
C8088 |
| Okadaic acid sodium salt from Prorocentrum concavum, minimum 95% (HPLC) |
O7760 |
| Phenylarsine oxide, minimum 97%, Powder |
P3075 |
| Phosphatase Inhibitor Cocktail 1, DMSO solution |
P2850 |
| Phosphatase Inhibitor Cocktail 2, Aqueous solution |
P5726 |
| Sodium fluoride solution, minimum 99% |
S1504 |
| Phosphatase Detection Kit |
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| Product Name |
Product # |
| Protein Tyrosine Phosphatase (PTP) Assay Kit, Non-Radioactive |
PTP-101 |
| Reagents and Standards |
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| Product Name |
Product # |
| Ammonium molybdate tetrahydrate, ACS Reagent, 81.0-83.0% as MoO3 |
A7302 |
| Barium hydroxide octahydrate, minimum 98.0% |
B2507 |
| a-Casein from bovine milk, minimum 70% as as-casein (electrophoresis), Lyophilized powder |
C6780 |
| b-Casein from bovine milk, minimum 90% as b-casein (electrophoresis), Essentially salt-free, lyophilized powder |
C6905 |
| Albumin from chicken egg white, Grade V, minimum 98% (agarose gel electrophoresis), Lyophilized powder, ovalbumin |
A5503 |
| Phosvitin from egg yolk |
P1253 |
| Iron(II) sulfate heptahydrate, minimum 99.0%, (Ferrous sulfate) |
F7002 |
| Sodium phenyl phosphate dibasic dihydrate, 95%, (Phenylphosphate) |
P7751 |
| Phosphorous Standard |
661-9 |
| Phosphopeptide positive control set |
P9615 |
| Malachite Green hydrochloride |
M9636 |
| Fiske-SubbaRow Reducer |
661-8 |
| Serine/Threonine Kinase Inhibitors |
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| Product Name |
Product # |
| 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7) |
H-121 |
| ML-7, Powder |
I2764 |
| PD 98,059, Solid |
P-215 |
| SB 203770, Powder |
S8307 |
| D-Sphingosine, bovine brain, approx. 99% (TLC) |
S6879 |
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