Phosphorylation

Elution Conditions

Product No: P 1617

Plate Enrichment - HPLC

Table 1 Elution Conditions
Elution Solutions Comments
0.2 M sodium phosphate, pH 8.4 Provides competitive displacement. Requires salt removal for MALDI-TOF-MS.
0.15 to 0.4 M ammonium hydroxide Provides significant recovery of phosphocompounds. Compatible with MALDI-TOF-MS.
0.15 M ammonium hydroxide with 25% acetonitrile Provides significant recovery of phosphocompounds. Compatible with MALDI-TOF-MS.
1 to 5% TFA in water Provides significant recovery of monophosphocompounds. Compatible with MALDI-TOF-MS.