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Life Science > Proteomics > Post Translational Analysis > Protein Phosphorylation > Elution Conditions
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Phosphorylation
Elution Conditions | ||
Plate Enrichment - HPLC
| Table 1 Elution Conditions | |
| Elution Solutions | Comments |
| 0.2 M sodium phosphate, pH 8.4 | Provides competitive displacement. Requires salt removal for MALDI-TOF-MS. |
| 0.15 to 0.4 M ammonium hydroxide | Provides significant recovery of phosphocompounds. Compatible with MALDI-TOF-MS. |
| 0.15 M ammonium hydroxide with 25% acetonitrile | Provides significant recovery of phosphocompounds. Compatible with MALDI-TOF-MS. |
| 1 to 5% TFA in water | Provides significant recovery of monophosphocompounds. Compatible with MALDI-TOF-MS. |