Phosphorylation

Enzymatic Protein Deglycosylation Kit

 
The E-DEGLY Kit contains all the enzymes and reagents needed to completely remove all N-linked and simple O-linked carbohydrates from glycoproteins, as well as cleave complex core O-linked carbohydrates including those containing polylactosamine.

Features & Benefits
  • Single reaction at neutral pH
    No need to adjust conditions for each glycosidase reaction
  • Native & denaturing procedures
    Allows flexibility to ensure resulting deglycosylated proteins are compatible with down-stream analysis
  • Uses highly purified glycosidases, free of contaminating proteases
    No degradation of protein results in clear, reliable results
  • Removes all N-linked and O-linked sugars containing polysialic acid
    Results in more complete deglycosylation
  • Includes fetuin which has sialylated N- and O-linked oligosaccharides
    Provides a control to check for efficiency of the deglycosylation
  • Each reaction deglycosylates 200 µg of glycoprotein
    Yields enough deglycosylated protein for further analysis
  • Contains sufficient reagent for the deglycosylation of approx. 2 mg of protein
    Allows for the processing of multiple samples

Kit Components
    PNGase F
    Endo-O-Glycosidase
    a-2(3,6,8,9)-Neuraminidase (Sialidase A)
    ß(1,4)-Galactosidase
    ß-N-Acetylglycosaminidase
    5X Reaction Buffer
    Denaturation Solution
    Triton™ X-100 (15% solution)
    Fetuin Control

In contrast to the In-gel Deglycosylation Kit, this kit is optimized for the removal of glycans from proteins in solution. This can simplify electrophoretic analysis by reducing the heterogeneity normally associated with glycoproteins. The kit is also useful if the protein(s) of interest are not separated by electrophoresis but analyzed by other means.
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