Enzymatic Protein Deglycosylation Kit

The E-DEGLY Kit contains all the enzymes and reagents needed to completely remove all N-linked and simple O-linked carbohydrates from glycoproteins, as well as cleave complex core O-linked carbohydrates including those containing polylactosamine.

Features & Benefits

Single reaction at neutral pH
No need to adjust conditions for each glycosidase reaction
Native & denaturing procedures
Allows flexibility to ensure resulting deglycosylated proteins are compatible with down-stream analysis
Uses highly purified glycosidases, free of contaminating proteases
No degradation of protein results in clear, reliable results
Removes all N-linked and O-linked sugars containing polysialic acid
Results in more complete deglycosylation
Includes fetuin which has sialylated N- and O-linked oligosaccharides
Provides a control to check for efficiency of the deglycosylation
Each reaction deglycosylates 200 µg of glycoprotein
Yields enough deglycosylated protein for further analysis
Contains sufficient reagent for the deglycosylation of approx. 2 mg of protein
Allows for the processing of multiple samples

Kit Components
    PNGase F
    Endo-O-Glycosidase
    a-2(3,6,8,9)-Neuraminidase (Sialidase A)
    ß(1,4)-Galactosidase
    ß-N-Acetylglycosaminidase
    5X Reaction Buffer
    Denaturation Solution
    Triton™ X-100 (15% solution)
    Fetuin Control

In contrast to the In-gel Deglycosylation Kit, this kit is optimized for the removal of glycans from proteins in solution. This can simplify electrophoretic analysis by reducing the heterogeneity normally associated with glycoproteins. The kit is also useful if the protein(s) of interest are not separated by electrophoresis but analyzed by other means.

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