Phosphorylation Analysis Tools

Tools for success in analyzing phosphorylation targets!

Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded by the human genome contain covalently bound phosphate. The 'average' protein kinase can add phosphates to 20 different proteins and the 'average' protein phosphatase removes phosphate from 60 different proteins.

Thirty percent of drug discovery programs and R&D investment by the pharmaceutical industry targets protein kinases. Only recently did the research community fully appreciate protein phosphorylation, adopting that protein kinases and protein phosphatases represented valid drug targets. Up to sixty kinase inhibitors have undergone clinical evaluation against cancers, inflammation, diabetes and neurodegenerative diseases.

Capture of Phosphorylated Proteins and Peptides

PHOS-Select^TM
An exceptional efficient chemistry for phosphopeptide enrichment

• High capacity (at least 4 μmoles of phosphopeptide per mL of resin)
• Precharged with iron (III) as a stable chelate
• Provides a single-step phosphocompound purification

Supel-Tips for Phosphopeptide Enrichment
Titania & Zirconia-silica composite pipette tips

• Superior recovery and reproducibility
• Exceptional binding capacity and enhanced affinity
• Effective at achieving high capacity for trace level peptides
• Excellent sorbent bed stability for cleaner samples
• Fast and effective analyte retention/elution
• Properties –
  • Proprietary composite particles and adhesives
  • 50-60 μm particle size, 300 Å pore size

Capture Media for Phosphoproteins

Identification of Phosphorylated Proteins

PhosDecor^TM
Quick and selective identification of phosphorylated protein directly in SDS-polyacrylamide gels. Particularly useful in screening for phosphoproteins in complex protein samples.

• Complete in as little as 4 hours
• Gels may be dual stained for total protein
• Linear detection of phosphoproteins over a wide range
• Compatible with downstream analyses such as in-gel trypsin digestion and MALDI-MS analysis

Reagents and Controls for Phosphoprotein Analysis

Dephosphorylation for Downstream Analysis

Beta Elimination Kit

• Specific O-linked removal
• Preserves both the protein and the PTM

Enzymatic Deglycosylation Kit

• Single reaction at neutral pH
• Native and denaturing procedures allow flexibility to ensure resulting proteins are compatible with down-stream analysis
• Sufficient reagent for approximately 2 mg of protein

Native Protein Deglycosylation Kit

• Enzymes included are less sensitive to protein conformation than PNGase Fc
• Sufficient reagent for approximately 2 mg of protein

In-gel Deglycosylation Kit

• Conveniently prepares protein samples for analysis by MS or HPLC.
• Proteins remain intact
• Highly purified enzymes possess no unwanted activities or additives to complicate analysis
• Works in solution or with gel slices

Related Products and Topics

Phosphorylation Analysis Products
Phosphatase Inhibitors
Phosphorylation References
Glycobiology
Antibody Explorer