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Life Science > Proteomics > Post Translational Analysis > Protein Phosphorylation > Proteoprofile™ Kit
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Protein Phosphorylation
Proteoprofile™ Kit | ||
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Most proteins in eurkaryotic cells undergo post-translational modification, quite commonly glycosylation. Unfortunately, this type of modification leads to problems in subsequent protein analysis procedures. Glycopeptides generally do not readily ionize during MS analysis leading to insufficient spectral data. Furthermore, proteolytic digestion of the native glycoprotein is often incomplete due to steric hindrance from the bulky oligosaccharides attached to the protein molecule. Hence removal of the carbohydrate groups from a glycoprotein prior to protein identification is preferred. |
| Sigma's ProteoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient and reproducible method to N-deglycosylate and tryptically digest protein samples from 1D or 2D polyacrylamide gel slices for subsequent MS or HPLC analysis. The procedure is suitable for Coomassie Blue and Colloidal Coomassie stained gels and may be used with gels silver stained and destained using Sigma's Proteo Silver™ Plus kit ( PROT-SIL2). ProteoProfile Enzymatic In-gel N-Deglycosylation kit includes the enzymes and reagents necessary for N-linked deglycosylation and tryptic digestion. The samples can then be desalted and concentrated for analysis by MALDI-TOF or electrospray MS. |
| Kit Components Destaining Solution Proteomics Grade PNGase F Proteomics Grade Trypsin Trypsin Solubilization Reagent Trypsin Reaction Buffer Ribonuclease B (positive control) Peptide Extraction Solution Biotech Grade Acetonitrile |
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Features & Benefits
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| View Protocol Diagram |
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