Product No: S 6940 (96-well, clear)
Product No: S 8815 (384-well, clear)
Selectivity of Binding on Streptavidin HC Plate
Figure 1: MALDI-MS analysis of trypsin digested biotinylated lysozyme introduced to a Streptavidin HC plate from ion-exchange effluent of HPLC. A) supernatant of fraction 8 collected in plates, B) peptides eluted from Streptavidin HC plates using 10 mg/ml ACCA matrix in 70% acetonitrile, 0.1% formic acid. The peptides were introduced to the wells as fractions eluted from SCX ion-exchange HPLC in 25% acetonitrile 10 mM ammonium phosphate pH 3 with increasing amounts of 0.5 M ammonium chloride. The spectra show peptides eluted from a single well. The biotinylated peptides are designated in green.
Comparison of Streptavidin HC Plate to Monomeric Avidin Agarose
Figure 2: (A) MALDI-MS analysis of trypsin digested biotinylated lysozyme prior to loading on either B) Streptavidin HC plates; or C) monomeric avidin agarose. Spectra B and C show purified biotinylated peptides eluted using 10 mg/ml ACCA matrix in 70% acetonitrile, 0.1% formic acid. Samples were loaded on the appropriate affinity matrix in 25% acetonitrile, 10 mM ammonium phosphate, 0.5 M ammonium chloride pH 3 at 2 nmole/ml. The biotinylated peptides are designated in green.
ACCA: a-cyano-4-hydroxycinnamic acid
| Peptide Profile of Trypsin Digested Lysozyme |
| Designation |
From-To |
Sequence |
Mass (MH+) |
| T18 |
129-129 |
L |
132.1809 |
| T1 |
1-1 |
K |
147.1956 |
| T12 |
97-97 |
K |
147.1956 |
| T14 |
14-14 |
R |
175.2091 |
| T2 |
2-5 |
VFGR |
478.5654 |
| T10 |
69-73 |
TPGSR |
517.5569 |
| T15 |
115-116 |
CK |
663.8523 |
| T17 |
126-128 |
GCR |
748.9172 |
| T3 |
15-21 |
HGLDNYR |
874.9210 |
| T16 |
117-125 |
GTDVQAWIR |
1046.1578 |
| T3 |
5-13 |
CLLAAAMK |
1250.5550 |
| T9 |
62-68 |
WWCNDGR |
1350.5273 |
| T7 |
34-45 |
FESNFNTQATNR |
1429.4715 |
| T13 |
98-112 |
IVSDGNGMNAWVAWR |
1676.8748 |
| T6 |
22-33 |
GYSLGNVVVCAAK |
1682.9631 |
| T8 |
46-61 |
NTDGSTDYGILQINSR |
1754.8303 |
| T11 |
74-96 |
NLCNPCSALLSSDITASVNCAK |
3579.2433 |
Table 1: Theoretical peptide fragments from trypsin digestion of lysozyme. The calculated mass values include the MW of the biotinylation reagent for the cysteine containing residues. The biotinylated peptides are designated in red. |
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