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Protein Chromatography

Analytical HPLC

Sigma-Aldrich offers a wide variety of analytical scale High Performance Liquid Chromatography (HPLC) columns for the proteomics researcher. Many of these columns are compatible to LC-MS applications.

Our HPLC columns enable you to optimize your separation with respect to retention, resolution, selectivity, and analysis time, all while minimizing method development time. Separations are completely scalable from analytical to preparative.

LC-MS Columns
Discovery and Discovery BIO column specifications
Ascentis and Ascentis Express column specifications


Discovery column features
Ascentis column features
Ascentis Express column features


Discovery Columns:

 

Discovery® C18
Discovery® C8
Discovery® Cyano
Discovery® HS F5
Discovery® BIO Wide Pore C18
Discovery® BIO Wide Pore C8
Discovery® BIO Wide Pore C5 
Discovery® BIO GFC

 


Ascentis Columns:

 

Ascentis® C18
Ascentis® C8
Ascentis® Silica
Ascentis® Express C18
Ascentis® Express C8
Ascentis® Express HILIC

 


Literature and Tools:

 


On-line Product Catalog

Discovery is a registered trademark of Sigma-Aldrich.
Ascentis is a registered trademark of Sigma-Aldrich.
TSK-GEL is a registered trademark of TosoH Corp.

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Discovery and Discovery BIO Column Specifications


 

 

  C18 C8 Cyano HS C18 HS F5 BIO Wide
Pore C18
BIO Wide
Pore C8
BIO Wide
Pore C5
Bonded Phase Octadecylsilane Octylsilane Cyanoprpyl Octadecylsilane Pentafluoro-phenylpropyl Octadecylsilane Octylsilane Pentylsilane
Endcap (yes/no) Yes Yes Yes Yes Yes Yes Yes Yes
Particle Platform Silica Silica Silica Silica Silica Silica Silica Silica
Particle Purity <10 ppm
metals
<10 ppm
metals
<10 ppm
metals
<10 ppm
metals
<10 ppm
metals
<10 ppm
metals
<10 ppm
metals
<10 ppm
metals
Particle Size (Å) 180 180 180 120 120 300 300 300
Surface Area (m2/g) 200 200 200 300 300 100 100 100
%C 12 7.5 4.5 20 12 ~9 ~5 ~3.5
Coverage (µmoles/m2) 3 3.4 3.5 3.8   ~3.6 ~4 ~4.5
pH Range 2–8 2–8 2–8 2–8 2–8 2–8* 2–8* 2–8*
Temperature Range Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C
*Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer.

 

Visit our complete listings of Discovery and Discovery BIO Wide products

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Ascentis and Ascentis Express Column Specifications


 

 

  Ascentis C18 Ascentis C8 Ascentis Silica Ascentis Express C18 Ascentis Express C8 Ascentis Express HILIC
Bonded Phase Octadecyl Octyl None Monomeric Monomeric Monomeric
Endcap (yes/no) Yes Yes No Yes Yes Yes
Particle Platform Type B silica Type B silica Type B silica Type B silica Type B silica Type B silica
Particle Purity <5 ppm
metals
<5 ppm
metals
<5 ppm
metals
     
Particle Size (Å) 100 100 100 90 90 90
Surface Area (m2/g) 450 450 450 150 150 150
%C 25 15 0      
pH Range 2–8* 2–8* 2–6 2–9 2–9 2–9
Temperature Range Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C Up to 70 °C
*Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer.

 

Visit our complete listings of Ascentis and Ascentis Express products

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