Analytical HPLC

Sigma-Aldrich offers a wide variety of analytical scale High Performance Liquid Chromatography (HPLC) columns for the proteomics researcher. Many of these columns are compatible to LC-MS applications.

Our HPLC columns enable you to optimize your separation with respect to retention, resolution, selectivity, and analysis time, all while minimizing method development time. Separations are completely scalable from analytical to preparative.

LC-MS Columns
Discovery and Discovery BIO column specifications
Ascentis and Ascentis Express column specifications

Discovery column features
Ascentis column features
Ascentis Express column features

Discovery Columns:

Discovery® C18
Discovery® C8

Discovery® Cyano
Discovery® HS F5

Discovery® BIO Wide Pore C18
Discovery® BIO Wide Pore C8
Discovery® BIO Wide Pore C5
Discovery® BIO GFC

Ascentis Columns:

Ascentis® C18
Ascentis® C8
Ascentis® Silica

Ascentis® Express C18
Ascentis® Express C8

Ascentis® Express HILIC

TSK-GEL® HPLC Columns

Literature and Tools:

Discovery BIO Wide Pore Brochure (532 Kb PDF)
Getting started with RP-HPLC for Peptides (69 Kb PDF)
Eliminate TFA and Improve Sensitivity of Peptide Analysis by LC-MS (58 Kb PDF)
Effect of Stationary Phase on Selectivity of Reverse-Phase HPLC Separations of Polypeptides (75 Kb PDF)
Discovery HPLC Columns for Small Molecule Separations (1.2 Mb PDF)
Ascentis HPLC Column Brochure (2.13 Mb PDF)
Ascentis Application Index
Developing HPLC Methods on Ascentis
Ascentis Express Brochure (1.05 Mb PDF)
HPLC Web Seminars

On-line Product Catalog

Discovery is a registered trademark of Sigma-Aldrich.
Ascentis is a registered trademark of Sigma-Aldrich.
TSK-GEL is a registered trademark of TosoH Corp.

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Discovery and Discovery BIO Column Specifications

	C18	C8	Cyano	HS C18	HS F5	BIO Wide Pore C18	BIO Wide Pore C8	BIO Wide Pore C5
Bonded Phase	Octadecylsilane	Octylsilane	Cyanoprpyl	Octadecylsilane	Pentafluoro-phenylpropyl	Octadecylsilane	Octylsilane	Pentylsilane
Endcap (yes/no)	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Particle Platform	Silica	Silica	Silica	Silica	Silica	Silica	Silica	Silica
Particle Purity	<10 ppm metals	<10 ppm metals	<10 ppm metals	<10 ppm metals	<10 ppm metals	<10 ppm metals	<10 ppm metals	<10 ppm metals
Particle Size (Å)	180	180	180	120	120	300	300	300
Surface Area (m2/g)	200	200	200	300	300	100	100	100
%C	12	7.5	4.5	20	12	~9	~5	~3.5
Coverage (µmoles/m2)	3	3.4	3.5	3.8		~3.6	~4	~4.5
pH Range	2–8	2–8	2–8	2–8	2–8	2–8*	2–8*	2–8*
Temperature Range	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C
*Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer.

Visit our complete listings of Discovery and Discovery BIO Wide products

Ascentis and Ascentis Express Column Specifications

	Ascentis C18	Ascentis C8	Ascentis Silica	Ascentis Express C18	Ascentis Express C8	Ascentis Express HILIC
Bonded Phase	Octadecyl	Octyl	None	Monomeric	Monomeric	Monomeric
Endcap (yes/no)	Yes	Yes	No	Yes	Yes	Yes
Particle Platform	Type B silica	Type B silica	Type B silica	Type B silica	Type B silica	Type B silica
Particle Purity	<5 ppm metals	<5 ppm metals	<5 ppm metals
Particle Size (Å)	100	100	100	90	90	90
Surface Area (m2/g)	450	450	450	150	150	150
%C	25	15	0
pH Range	2–8*	2–8*	2–6	2–9	2–9	2–9
Temperature Range	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C	Up to 70 °C
*Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer.

Visit our complete listings of Ascentis and Ascentis Express products

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