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Sigma-Aldrich offers a wide variety of analytical scale High Performance Liquid Chromatography (HPLC) columns for the proteomics researcher. Many of these columns are compatible to LC-MS applications.
Our HPLC columns enable you to optimize your separation with respect to retention, resolution, selectivity, and analysis time, all while minimizing method development time. Separations are completely scalable from analytical to preparative.
LC-MS Columns
Discovery and Discovery BIO column specifications
Ascentis and Ascentis Express column specifications
Discovery column features
Ascentis column features
Ascentis Express column features
Discovery Columns:
Ascentis Columns:
Literature and Tools:
On-line Product Catalog
Discovery is a registered trademark of Sigma-Aldrich.
Ascentis is a registered trademark of Sigma-Aldrich.
TSK-GEL is a registered trademark of TosoH Corp.
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Discovery and Discovery BIO Column Specifications
| |
C18 |
C8 |
Cyano |
HS C18 |
HS F5 |
BIO Wide
Pore C18 |
BIO Wide
Pore C8 |
BIO Wide
Pore C5 |
| Bonded Phase |
Octadecylsilane |
Octylsilane |
Cyanoprpyl |
Octadecylsilane |
Pentafluoro-phenylpropyl |
Octadecylsilane |
Octylsilane |
Pentylsilane |
| Endcap (yes/no) |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
| Particle Platform |
Silica |
Silica |
Silica |
Silica |
Silica |
Silica |
Silica |
Silica |
| Particle Purity |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
<10 ppm
metals |
| Particle Size (Å) |
180 |
180 |
180 |
120 |
120 |
300 |
300 |
300 |
| Surface Area (m2/g) |
200 |
200 |
200 |
300 |
300 |
100 |
100 |
100 |
| %C |
12 |
7.5 |
4.5 |
20 |
12 |
~9 |
~5 |
~3.5 |
| Coverage (µmoles/m2) |
3 |
3.4 |
3.5 |
3.8 |
|
~3.6 |
~4 |
~4.5 |
| pH Range |
2–8 |
2–8 |
2–8 |
2–8 |
2–8 |
2–8* |
2–8* |
2–8* |
| Temperature Range |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
| *Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer. |
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Ascentis and Ascentis Express Column Specifications
| |
Ascentis C18 |
Ascentis C8 |
Ascentis Silica |
Ascentis Express C18 |
Ascentis Express C8 |
Ascentis Express HILIC |
| Bonded Phase |
Octadecyl |
Octyl |
None |
Monomeric |
Monomeric |
Monomeric |
| Endcap (yes/no) |
Yes |
Yes |
No |
Yes |
Yes |
Yes |
| Particle Platform |
Type B silica |
Type B silica |
Type B silica |
Type B silica |
Type B silica |
Type B silica |
| Particle Purity |
<5 ppm
metals |
<5 ppm
metals |
<5 ppm
metals |
|
|
|
| Particle Size (Å) |
100 |
100 |
100 |
90 |
90 |
90 |
| Surface Area (m2/g) |
450 |
450 |
450 |
150 |
150 |
150 |
| %C |
25 |
15 |
0 |
|
|
|
| pH Range |
2–8* |
2–8* |
2–6 |
2–9 |
2–9 |
2–9 |
| Temperature Range |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
Up to 70 °C |
| *Recommended range is pH 2–8 but higher pH values are allowable using organic base buffer. |
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