Proteomics

Protein Electrophoresis

In modern life sciences, electrophoresis is s fundamental technique for separation, isolation and characterization of all kinds of biomolecules. In most cases, it has become an easy, uncomplicated and fast analysis method, used in wide variety of applications from protein to nucleic acid research. Electrophoresis can be used for monitoring the result of single critical steps before proceeding to the next part of the protocol, as well for the final analysis of the experiment.

In protein electrophoresis, reseachers have access to techniques such as native or SDS polyacrylamide electrophoresis or electrophoresis separation, based on differences in isoelectric points (IEF). With the growing need to analyse an ever increasing number of unknown gene products, multidimensional analysis, such as 2D electrophoresis, is popular.

Polyacrylamide gel electrophoresis (PAGE) is a technique Sigma supports with a selection of MW Markers for electrophoresis spanning a PAGE mass range from 1,060 to 545,000 daltons, silver stain markers, fluorescent markers, biotinulated markers, recombinant markers, brightly colored markers, and many more MW Markers for PAGE electrophoresis.