Protein Electrophoresis

Molecular Weight Markers

Protein molecular weight markers are used to calculate sample molecular weights, to monitor the progress of an electrophoretic run, or as a positive control for analysis conditions. Sigma offers a wide selection of markers for numerous protein electrophoresis applications, including silver staining, isoelectric focusing, fluorescent studies, and many more. All have been use-tested to assure outstanding performance and are available in convenient package sizes.

 ColorBurst™ Markers

Brilliantly colored, exceptionally well-resolved, convenient, and stable, ColorBurst protein molecular weight marker performs impressively in a variety of gel compositions and concentrations. ColorBurst is composed of eight proteins ranging from 8 to 220 kDa, which have been chemically reduced, alkylated, and conjugated to brilliantly colored dyes. ColorBurst can be used to estimate sample molecular weights, to monitor the progress of an electrophoretic run, or to confirm that an electroblot is complete. Each vial contains 500 ml of marker solution and may be used for up to 100 applications.

Features & Benefits

  • Exceptional resolution allows estimation of sample molecular weights
  • No freeze / thaw cycle means decreased degradation and long shelf life
  • Ready-to-use No boiling required before use
  • No chemical reduction necessary prior to gel loading
ColorBurst Marker 10-20% Tris-Glycine

C1992
A 10-20% Tris-Glycine gel was loaded (left to right) with 3, 5 and 7 µl of the ColorBurst Marker. The markers were run using standard conditions on 10 x 10 cm, 1 mm thick, 10-well precast gels.

Bands transferred to nitrocellulose membranes from the gels in Figure 1 (Tris-Glycine on left and Tris-Tricine on right). Transfers were completed in 90 minutes at 70 volts with Towbin's buffer (Tris-Glycine in 20% methanol.)

ColorBurst Marker 4-20% Tris-Glycine

C4861
The gel was loaded (left to right) with 3, 5, and 7 µl of ColorBurst Marker. The marker was run using standard conditions on a 10 x 10 cm, 1 mm thick, 10 well precast 4-20% Tris-Glycine gel.

Bands were transferred to nitrocellulose membrane. The transfer was completed in 90 minutes at 70 volts using Towbin’s buffer (Tris-Glycine in 20% methanol).

 

Product No. Product Name Size
C1992 ColorBurst™ Marker Electrophoresis Marker (M.W. 8,000-220,000) 1 vial
C4861 ColorBurst™ Marker Electrophoresis Marker, High Range (M.W. 30,000-220,000) 1 vial

 Color Markers

Sigma Color Markers contain 5-6 proteins conjugated to colored dies providing a monitor for protein migration and transfer efficiency. These markers are available in low range, suitable for use in the Laemmli SDS-PAGE system and ultra-low range for use in a Tricine SDS-PAGE system.

C6210 was separated
on a 10-20% Tris-tricine gel.
    
C6210 Color Marker Ultra-low Range (M.W. 1,060-26,600)   C3187 Color Marker Low Range (M.W. 6,500-45,000) C3187 was separated
on a 16% Tris-tricine gel.

 

Product No. Product Name Size
C6210 Color Marker Ultra-low Range (M.W. 1,060-26,600) 1 vial
C3187 Color Marker Low Range (M.W. 6,500-45,000) .5 mL

 Prestained Blue Marker

Sigma’s prestained blue consists of 7 proteins conjugated to a blue dye. This marker provides a monitor for protein migration and transfer efficiency.

SDS7B2 Prestained Molecular Weight Marker (M.W. 27,000-180,000)

SDS7B2 was separated on a 4-20% Tris-glycine gel.

 

Product No. Product Name Size
SDS7B2 Prestained Molecular Weight Marker (M.W. 27,000-180,000) 1 vial

 SigmaMarkers™

SigmaMarkers encompass the range of molecular weights common to most proteins and their subunits. Lyophilized with sample buffer, the wide, high, and low range markers are ready for use following reconstitution with water. They are formulated to yield a distribution of well- defined bands of approximately equal intensity following electrophoresis and Coomassie® Blue staining.
 

Product No. Product Name Size
S8445 SigmaMarker Wide Range (M.W. 6,500-200,000) 1 & 10 vial
M3913 SigmaMarker™ Low Range (M.W. 6,500-66,000) 1 vial
S8320 SigmaMarker High Range (M.W. 36,000-200,00) 1 & 10 vial

 Ultra-Low Range Marker

Sigma Molecular Weight Markers are available in ultra-low range weight ranges. The ultra-low range marker is suitable for use in a Tricine SDS-PAGE system.

Ultra Low Range Marker run on a 10-20% Tris-Tricine SDS-PAGE gel 5 µl of a 1:20 dilution of the Ultra-low Range Molecular Weight Marker (M3546) was run on a 10-20% Tris-Tricine SDS-PAGE gel, fixed with 5% gluteraldehyde and stained with EZBlue (G1041).
Product No. Product Name Size
M3546 Ultra-Low Range Molecular Weight Marker (M.W. 1,060-26,600) 1 vial

 Peptide Marker

The Peptide Molecular Weight Marker is a mixture of seven polypeptides ranging for 2,500 to 17,000 Da.
 

Product No. Product Name Size
MWSDS17S Peptide Molecular Weight Marker (M.W. 2,500-17,000) 1 vial

 Other Unstained Molecular Weight Markers

Sigma’s long standing markers, SDS7 and SDS6H2 previously titled Dalton markers, contain a mixture of proteins suitable for use in both the Weber Osborn (1) Electrophoresis System and the Laemmmli SDS-PAGE system.

SDS7 was separated on a
4-20% Tris-glycine gel and
stained with EZ Blue Protein Stain.

SDS7 Molecular Weight Marker (M.W. 14,000-66,000)

 

SDS6H2 Molecular Weight Marker (M.W. 30,000-200,000)

SDS6H2 was separated on a
4-20% Tris-glycine gel and
stained with EZ Blue Protein Stain.

 

Product No. Product Name Size
SDS7 Molecular Weight Marker (M.W. 14,000-66,000) 1 vial
SDS6H2 Molecular Weight Marker (M.W. 30,000-200,000) 1 vial

1. Weber, K.; Osborn, M. J. Biol. Chem. 244:4406, (1969)

 Chemichrome™ Western Control

Chemichrome Western Control is an ideal molecular weight marker and positive control for electrophoresis and subsequent Western blotting. Similar to ColorBurst™, Chemichrome contains an additional band of mouse IgG. During electrophoresis, brightly colored protein bands serve as positive controls for protein migration. During blotting, the same brightly colored bands indicate that the transfer is complete. After incubation with mouse primary and secondary antibodies, the band of mouse IgG (heavy chain) serves as a positive control using either colorimetric or chemiluminescent substrates. Simple to use and extremely practical, Chemichrome Western Control enables researchers to confirm the success of their analyses every step of the way. Chemichrome is supplied in vials containing 200 µl of ready-to-use solution.

Features & Benefits

  • Confirms that a membrane transfer is complete
  • Confirms successful Western Blotting conditions
  • Compatible with many peroxidase and phosphatase substrates, including TMB and ECL®
Product No. Product Name Size
C2242 Chemichrome™ Western Control 1 vial

 Chemichrome™ Ultimate

Like Colorburst™ and Chemichrome™ Western Control, Chemichrome Ultimate contains eight brilliantly colored polypeptides which serve as positive controls during protein migration and transfer. In addition to mouse IgG, Chemichrome Ultimate also contains goat and rabbit IgG, which serve as positive controls for Western blotting using either colorimetric or chemiluminescent substrates.
 

Product No. Product Name Size
C2117 Chemichrome™ Ultimate 1 vial

 ProteoProfile™ PTM Marker

Glycosylation and phosphorylation are frequently encountered post-translation modifications in proteomic analysis. ProteoProfile PTM Marker, containing glycosylated and phosphorylated proteins, is designed for use as a positive and negative control in studying these post- translational modifications of proteins in SDS-PAGE. Electrophoresis will yield four protein bands:

Superior Selectivity ProteoProfile PTM Marker (P1745), containing glycosylated and non-glycosylated proteins, was separated by electrophoresis on a 4-20% SDS-PAGE gel. The gel was stained with GlycoProfile III (PP0300) (left), fluorescently imaged, and then stained for total protein with EZBlue™ Gel staining reagent (G1041) (right). Each band represents approximately 300 ng of protein.


  1. BSA (not glycosylated, not phosphorylated)
  2. Ovalbumin (glycosylated and phosphorylated)
  3. b-Casein (not glycosylated, phosphorylated)
  4. RNase B (glycosylated, not phosphorylated)

 

Product No. Product Name Size
P1745 ProteoProfile PTM Marker 0.1 ml

 Biotinylated Markers

Biotinylated molecular weight markers contain biotin-conjugated proteins which, following electrophoresis and membrane transfer, can be easily detected with a Streptavidin-Peroxidase Conjugate (S5512) and a color development reagent such as TMB (T0565).

B2787 Biotinylated Molecular Weight Marker (M. W. 6,500-180,000)

B2787 was separated on a 12.5% gel transferred to a nitrocellulose membrane and stained using Streptavidin Peroxidase (S5512) and color reagent (C8890).

 

Product No. Product Name Size
B2787 Biotinylated Molecular Weight Marker (M. W. 6,500-180,000) 1 vial

 2D Electrophoresis Markers

Specifically designed for denaturing IEF and 2D electrophoresis, this molecular weight marker set contains four proteins selected to provide a diagonal pattern on a wide range 2D gel.

2D Gel Electrophoresis Marker containing amyloglucosidase, Ovalbumin, carbonic anhydrase and myoglobin

5 µl of 2D markers (M3411) were separated with a mixture of ampholine pH 3.5-9.5 and ampholine pH 2.5-4.5 in the ratio 1:2.5 and then separated on a 12.5% SDS-PAGE gel. The gel was stained with Coomassie Brilliant Blue G (B8522).

  1. Amyloglucosidase from Aspergillus niger (A8437)
  2. Ovalbumin (O4757)
  3. Carbonic Anhydrase from Human Erythrocytes (C4806)
  4. Myoglobin from Horse Heart (M7911)
Product No. Product Name Size
M3411 Marker for 2D Electrophoresis 17,000-89,000
7.6-3.8 pl

 Fluorescent Markers

Sigma fluorescent molecular weight markers are composed of FITC-conjugated proteins, which are well resolved and easily visualized with ultraviolet light in polyacrylamide gels or membranes.

F3526 Fluorescent Molecular Weight Marker (M.W. 20,100-205,000)

 

F3526 was separated on a 4-20% Tris-glycine gel and viewed under a UV light.

 

Product No. Product Name Size
F3526 Fluorescent Molecular Weight Marker (M.W. 20,100-205,000) 1 vial

 Recombinant Markers

The Recombinant Molecular Weight Standard Mixture contains seven precisely sized proteins, molecular weights 15, 25, 35, 50, 75, 100, and 150 kDa. Recombinant proteins form sharp bands for accurate sample MW calculations.
 

Product No. Product Name Size
M0671 Recombinant Molecular Weight Marker (M.W. 15,000-150,000) .5 mL

 Silver Stain Markers

Designed for molecular weight determinations on silver stained gels, Silver Stain SDS- PAGE molecular weight standard mixtures contain evenly distributed, well resolved proteins of equal intensity. Check out our ProteoSilver™ Stains.

Silver Stain Molecular Weight Marker gel image 7 µl of a 1:15 dilution of the Silver Stain Molecular Weight Marker (M6539)
was run on a 10-18% SDS-PAGE gel and silver-stained.

 

Product No. Product Name Size
M6539 Silver Stain Molecular Weight Marker (M.W. 6,500-180,000) 1 vial

 SYPRO Marker

The SYPRO marker contains 11 proteins suitable for use in SDS-PAGE. Detection is completed with a SYPRO fluorescent protein gel stain.
 

Product No. Product Name Size
P7869 SYPRO® Molecular Weight Standard 1 vial

 Non-Denaturing Molecular Weight Marker

Sigma's Non-Denaturing Marker contains 5 separate proteins suitable for use in a native polyacrylamide gel electrophoresis system.
 

Product No. Product Name Size
MWND500 MWND500 Kit for Molecular Weights 14,000 - 500,000 Non-denaturing 1 KT

 IEF Marker (3.6-9.3)

The IEF Marker 3.6-9.3 is a mixture of proteins with well-defined pI values. The protein mixture forms evenly spaced, distinct bands over the designated pH range.
 

Product No. Product Name Size
I3018 IEF Mix 3.6-9.3 1 vial