Protein Electrophoresis

Western Blotting

Show us your ugliest blots. For a limited time, you can upload your ugly Western blot and we’ll provide tips and tricks for revealing beautiful Western data in our Protein Blotting Handbook, 6th edition.

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 Achieve Better Reproducibility, Signal-to-Noise Ratio, & Robustness

Western blotting is a well-established technique for detecting, analyzing, and quantifying proteins. However, Western blots often suffer from poor consistency, high background/low sensitivity, and lack of robustness. While blotting should be a science, it can seem like an unconquerable art. For streamlined Western blotting with high signal-to-noise ratios, think about the principles behind each step:

  1. Sample Preparation: use optimized extraction reagents and protease inhibitors; accurately quantify total protein
  2. Electrophoresis: obtain intact blots by using tear-resistant precast gels
  3. Transfer: use the correct transfer membrane that’s best for your analysis and detection method
  4. Immunodetection: optimize blocking and washing; use Western-tested antibodies and detection substrates of the right sensitivity.

 Rethink Western blotting!

See why you’ll get better outcomes when you use optimized tools for your Western blots.

Just getting started with Western blotting Analysis? 

Check out our Western Blot Literature & Resources to help guide you.
 

 Products for a Well-Thought-Out Western blotting Workflow

 Featured Solutions

 
Protease Inhibitors
Learn more about preserving protein functionality with well-established SigmaFAST™ and cOMPlete™ individual protease inhibitors and protease inhibitor cocktails. Or, click here to purchase.
 
 
Immobilon® Transfer Membrane
Choose a transfer membrane that gives you the best signal-to-noise ratio for your protein of interest.
   
 
 
TruPAGE™ Precast Gels
Learn more about how TruPAGE gels provide the band resolution you expect AND 10x the strength compared to competition. Or, click here to purchase.

 
 
SNAP i.d.® 2.0 System
Learn more about reducing the time for blocking, washing, and antibody probing to just 30 minutes, using the SNAP i.d.® 2.0 protein detection system. Request a demo.