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Western blotting or immunoblotting, is a process whereby proteins are transferred or "blotted" to a solid phase support, such as nitrocellulose or PVDF. Specific Proteins attached to the surface of the membrane are then detected through antibody-antigen reactions.
Nitrocellulose Membranes are the most popular matrix used in protein blotting. Immobilon™-P PVDF Membranes (Polyvinylidene Difluoride) membranes have been optimized for Western blotting and can offer better handling and staining than nitrocellulose. The new Immobilon™-FL PVDF membrane is the first transfer membrane optimized for fluorescence applications (low background) and is ideal for multiplexing and chemifluorescence applications.
QuickDraw™ blotting paper is used for Northern, Southern, and Western blots. Extrathick paper is ideal for Western blotting to sandwich the gel and the membrane. The thick paper may be used as a filter support for dot blots. Medium thickness paper is suitable for use as a wick during transfers. QuickDraw paper is available in a variety of sizes to meet your needs.
Nitrocellulose Membranes
Nitrocellulose membranes are the most popular matrix used in protein blotting. All of the following are suitable for Western blotting. Most proteins can be successfully blotted using a 0.45 µm pore size membrane. For proteins of low molecular weight or peptides, a 0.2 µm pore size membrane is recommended.
| Product # |
Product Name |
| N7892 |
Nitrocellulose Membrane 0.2 µm pore size, 15 x 15 cm |
| N8017 |
Nitrocellulose Membrane 0.2 µm pore size, 20 x 20 cm |
| N8142 |
Nitrocellulose Membrane 0.45 µm pore size, 7 x 10 cm |
| N8267 |
Nitrocellulose Membrane 0.45 µm pore size, 15 x 15 cm |
| N8392 |
Nitrocellulose Membrane 0.45 µm pore size, 20 x 20 cm |
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Immobilon™-P PVDF Membranes
Immobilon-P PVDF (Polyvinylidene Difluoride) membranes have been optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon-P PVDF membranes have a pore size of 0.45 µm.
| Product # |
Product Name |
| P2438 |
Immobilon-P PVDF Membrane 9 x 12 cm |
| P4188 |
Immobilon-P PVDF Membrane 10 x 10 cm |
| P2563 |
Immobilon-P PVDF Membrane 15 x 15 cm |
| P2813 |
Immobilon-P PVDF Membrane 26 x 26 cm |
| P2938 |
Immobilon-P PVDF Membrane 26.5 cm x 3.75 m |
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Immobilon™-FL PVDF Membrane
-The first transfer membrane optimized for fluorescence applications.
-Extremely low background improves sensitivity of all fluorescence detection protocols
-Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
-Ideal for multiplexing and chemifluorescence applications
| Product # |
Product Name |
| 05317 |
Immobilon™-FL PVDF membrane BioChemika, pore size 0.45 μm |
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QuickDraw™ Blotting Paper
QuickDraw blotting paper is used for Northern, Southern, and Western blots. Extrathick paper is ideal for Western blotting to sandwich the gel and the membrane. The thick paper may be used as a filter support for dot blots. Medium thickness paper is suitable for use as a wick during transfers. QuickDraw paper is available in a variety of sizes to meet your needs.
| Product # |
Product Name |
| P6664 |
Blotting Paper, Medium-Thick, 20 x 20 cm |
| P6914 |
Blotting Paper, Medium-Thick, 30 x 30 cm |
| P9039 |
Blotting Paper, Medium-Thick, 33 x 56 cm |
| P9164 |
Blotting Paper, Thick, 33 x 56 cm |
| P6928 |
Blotting Paper, Extra-Thick, 7 x 8 cm |
| P7796 |
Blotting Paper, Extra-Thick, 7 x 10 cm |
| P8046 |
Blotting Paper, Extra-Thick, 10 x 15 cm |
| P6803 |
Blotting Paper, Extra-Thick, 11 x 14 cm |
| P8549 |
Blotting Paper, Extra-Thick, 14 x 20 cm |
| P7921 |
Blotting Paper, Extra-Thick, 15 x 15 cm |
| P8171 |
Blotting Paper, Extra-Thick, 20 x 20 cm |
| P7176 |
Blotting Paper, Extra-Thick, 20 x 25 cm |
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