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Protein Quantitation

Bradford Reagent

The Bradford Reagent can be used to determine the concentration of proteins in solution. The procedure is based on the formation of a complex between the dye, Brilliant Blue G, and proteins in solution. The protein-dye complex causes a shift in the absorption maximum of the dye from 465 to 595 nm. The amount of absorption is proportional to the protein present. The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard assays. The linear concentration range is 0.1-1.4 mg/ ml of protein, using BSA (bovine serum albumin) as the standard protein. The Bradford Reagent is compatible with reducing agents, which are often used to stabilize proteins in solution. Other protein assay procedures (Lowry and BCA) are not compatible with reducing agents. The Bradford Reagent should be used in place of these protein assays if reducing agents are present. However, the Bradford reagent is only compatible with low concentrations of detergents (compatibility chart). If the protein sample to be assayed has detergents(s) present in the buffer, it is suggested to use the BCA protein determination procedure.

Features & Benefits

  • The reagent is ready to use. No mixing or dilution required.
  • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
  • Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
  • Reagent is suitable for micro (1- 10 mg/ml) and standard (50-1400 mg/ml) assays
  • Can be used in microwell plate assays

 

Ordering Information
Product #  Product Name Size
B6916 Bradford Reagent 500 mL

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