FluoroProfile Kit

Shed new light on your protein quantification.
FluoroProfile™ (Product No. FP0010) is significantly more sensitive than existing colorimetric methods, and exhibits a larger linear dynamic range than other fluorometric methods. Results are achieved in 20 minutes and reversible binding of the fluorophore allows for MS, HPLC, Edman degradation and other downstream assays. FluoroProfile also uses the water-soluble fluorophore epicocconone allowing for safe, economical disposal.
Sensitive - Detects over three orders of magnitude down to 40 ng/ml Simple - Results available in 20 minutes and stable for six hours Safe - Water soluble, biodegradable fluorophore enables safe, economical disposal

Experience these additional benefits:

Wide dynamic range, over 3-orders of magnitude - reduce dilution requirements prior to assay
Low protein-protein variability - improve confidence of accuracy for complex samples
No time-consuming heating and protein reduction steps - eliminates additional steps and speeds processing
Assay tolerates interfering compounds (DNA and solubilizing reagents) - eliminates additional steps, such as precipitation to remove buffers or detergents that may alter protein content
Stable for up to 6 hours - allows freedom to perform additional work concurrently
No strong solvents and acids - improves safe handling
No special instrument requirements - Designed for use in a wide range of fluorescence measuring instruments

Order Information

Product Code	Product Name	Size
FP0010	FluoroProfile Protein Quantification Kit	1 KT

Figure 1: Excitation and Fluorescence Emission Spectra

Figure 2: Typical Standard Curve

Figure 3: Fluorescence Signal Variation with Different Proteins

Interfering Compounds

Compound		Concentration
CHAPS		0.05%

SDS		0.1%

Thiourea		500 mM

EDTA		20 mM

NaCl		100 mM

TBP		10 mM

Urea		1 M

TCEP		2 mM

Glycerol		25%

Iodoacetamide		20 mM

2-Mercaptoethanol		20 mM

Sucrose		250 mM

Tris		500 µM

TRITON™ X-100		0.002 %

TWEEN®		0.005 %

Note: This is not a complete list of incompatible compounds. One may assay the protein of interest in ultrapure water alone, then in sample buffer with possible interfering substances. Comparison of the readings will indicate if interference exists. Alternatively, the interfering substance may be removed using dialysis or protein precipitation using the ProteoPrep® Protein Precipitation Kit (PROT-PR).

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