High Depletion Capacity

Figure 1: The novel antibody resin (PROT-IA) displays significantly higher binding capacity for human albumin and IgG than does the conventional antibody resin. The novel antibody resin can deplete 2-4 times more serum per ml of resin than the conventional resin. The novel antibody resin will deplete greater than 98% of albumin from 50 µl of serum whereas the conventional resin depletes less then 15 µl of serum (Panel A). The novel antibody resin will deplete greater than 98% of IgG from 50 µl of serum whereas the conventional resin depletes less then 20 µl of serum (Panel A).

High Specificity

Figure 2: The novel antibody resin (PROT-IA)displays higher specificity than a conventional antibody-based resin kit. The 2DE analysis software (Nonlinear Dynamics) detected 362 spots on the novel resin gel whereas 279 spots were detected on the conventional antibody resin gel. Ninety three (93) spots were detected on the novel resin gel which were not matched on the conventional resin gel. Only seven (7) spots were detected on the conventional gel not matched to the novel gel.

Removal of bound proteins and confirming specificity is easier

Figure 3: The bound proteins are more easily removed from the novel antibody resin (lanes 2, 3, 6 and 7) using IEF compatible reagents containing urea, thiourea and C7BzO detergent (Product Code C 0356). Following extraction with C 0356, the resins were boiled in Laemmli sample buffer containing sodium dodecyl sulfate (SDS) (Panel B, lanes 8 and 9). The linkage of the antibodies to the novel antibody resin appears more stable as displayed by the large amount of heavy and light chain antibody subunits cleaved from the conventional resin.

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