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Extraction Kits Reagents
ProteoPrep 20 LC
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ProteoPrep® 20 Plasma Immunodepletion LC Column (PROT20LC)
By removing 20 highly abundant proteins (representing 97% of the proteome) at 98% efficiency, less fractionation of the true plasma proteome is required; focusing on your protein biomarkers becomes faster and simpler!
- Reduce sample complexity and increase efficiency
- Reveal proteins previously masked
- Enrich proteins of interest up to 20-fold
Designed for use with LC instruments, the ProteoPrep® 20 LC column accommodates a sample load of 100 µL plasma or serum and can be used up to 100 times.
Read the press release:
Biomarker Discovery Simplified with Innovative Technology from Sigma-Aldrich
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Components
- 1 Depletion Column
- 10X Equilibration Buffer
- 10X Elution Buffer
- Kathon (for long-term storage)
- Collection Tubes
- Spin Filters
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Ordering Information
| Product # |
Product Name |
Package Size |
| PROT20LC |
ProteoPrep® 20 Plasma Immunoaffinity LC Column |
1 kit |
ProteoPrep® 20 Plasma Immunodepletion Technology
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The ProteoPrep® 20 technology offers the highest depletion of highly abundant plasma proteins available today and specifically removes 20 highly abundant proteins (representing 97% of the proteome) to reveal a new layer of the plasma proteome.
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| The ProteoPrep® 20 Technology depletes the following proteins: |
Albumin
IgGs
Transferrin
Fibrinogen
IgAs
α-2-Antitrypsin
IgMs
α-1-Antitrypsin
Complement C3
Haptoglobulin |
Apolipoprotein A1
Apolipoprotein A2
Apolipoprotein B
Acid-1-Glycoprotein
Ceruloplasmin
Complement C4
Complement C1q
IgDs
Prealbumin
Plasminogen |
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Twenty-two Unique Proteins Revealed with ProteoPrep® 20
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The ProteoPrep® 20 Plasma Immunodepletion Kit specifically removes 20 highly abundant proteins (representing 97% of the proteome) at 99% efficiency, revealing a new layer of highly abundant proteins. Initially, the gel image above appears to be similar to an undepleted sample, but identifications via mass spectrometry indicate that the visible proteins are not members of the list of twenty high abundance proteins specifically removed. They are, indeed, unique to a sample depleted with the ProteoPrep® 20 technology.
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| Spot No. |
Protein Identification
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1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22 |
Alpha-1-B-glycoprotein
Hemopexin (Beta-1B-glycoprotein)
Alpha-2-HS-glycoprotein
(Fetuin A)
Vitamin D Binding Protein
Pigment Epithelium-derived factor
Carboxypeptidase N
(Kininase 1)
Complement Factor H fragment
Apolipoprotein E
Ficolin 3 (Hakata antigen)
PK-120 (Plasma Kallikrein-sensitive Glycoprotein)
Apolipoprotein A-IV
Glutathione Peroxidase 3
Tetranectin (Plasminogen Binding Protein)
Serum amyloid A (SAA)
Kininogen
Complement Factor H-related protein 2
Inter-alpha-trypsin inhibitor H1
Vitronectin
Geltain-Binding Protein
Antithrombin III
Cluterin (Apolipoprotein J)
Inter-alpha-trypsin inhibitor H4 |
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References
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Resources
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Related Products
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ProteoPrep® 20 Plasma Immunodepletion Kit
ProteoPrep® 20 spin columns specifically remove 20 of the most abundant proteins from human plasma or serum to reveal previously masked protein biomarkers. Additionally, this facilitates a 20-fold increase in the relative amount of revealed proteins that can be subsequently studied and analyzed. Designed for use with bench top centrifuges, ProteoPrep® 20 spin columns can accommodate a sample load of 8 µL plasma or serum and can be used up to 100 times each. The ProteoPrep® 20 Plasma Immunodepletion Kit contains 3 spin columns. The ProteoPrep® 20 Sample Kit contains 1 spin column.
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Components
- Depletion Spin Column(s)
- 10X Equilibration Buffer
- 10X Elution Buffer
- Kathon (for long-term storage)
- Collection Tubes
- Spin Filters
- Syringes
- Luer Lock Caps
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| Product # |
Product Name |
Package Size |
| PROT20 |
ProteoPrep® 20 Plasma Immunodepletion Kit |
1 kit (3 columns) |
| PROT20S |
ProteoPrep® 20 Plasma Immunodepletion Sample Kit |
1 kit (1 column) |
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ProteoPrep® Blue Albumin & IgG Depletion Kit
ProteoPrep Blue Albumin Depletion Kit is designed to specifically remove albumin and IgG from 25 samples of human serum (25 µl to 100 µl) in preparation for two-dimensional electrophoresis (2DE). Albumin-depleted serum samples generated by this kit are in urea rather than salt-based buffers with little dilution, eliminating the need to precipitate the sample prior to performing 2DE.
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Components
- ProteoPrep® Blue Albumin and IgG Depletion Medium—a 10-ml suspension containing 60% packed medium
- ProteoPrep® Blue Equilibration buffer—1 bottle reconstitutes to 50 ml, pH 7.8
- Protein Extraction Reagent Type 4—1 bottle reconstitutes to 23 ml, pH 10.4
- 25 Spin columns
- 75 Collection Tubes
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| Product # |
Product Name |
Package Size |
| PROTBA |
ProteoPrep® Blue Albumin & IgG Depletion Kit |
1 kit |
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