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Immunoaffinity partitioning of highly-abundant proteins (HAP) has proven to be one of the most effective approaches for overcoming the wide dynamic range of plasma protein concentration, as well as enabling the detection of low-abundant proteins (LAP). Avian polyclonal IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that allow highly-specific partitioning of protein mixtures. Sigma offers a novel Seppro® IgY14 columns system, which takes you deeper than ever into the plasma proteome. IgY14 columns remove the top 14 HAP.
Proteins Partitioned by IgY-14 Microbeads:
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HSA
IgG
Fibrinogen
Transferrin
IgA
IgM
Haptoglobin
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alpha2-Macroglubulin
alpha1-Acid Glycoprotein
alpha1-Antitrypsin
Apo A-I HDL
Apo A-II HDL
Complement C3
LDL (ApoB)
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Our IgY14 systems are available in the following formats (suitable for your volumes):
| Product Name |
Column Use |
Human or
Primate plasma/serum |
Product # |
IgY14 Spin Column Kit
Includes 2 spin columns and reagents.
Good for 200 samples. |
100 times |
20µl |
SEP010 |
IgY14 LC-2 Column Kit
Includes 1 HPLC column and reagents. |
100 times |
50µl |
SEP020 |
IgY14 LC-5 Column Kit
Includes 1 HPLC column and reagents. |
100 times |
125µl |
SEP030 |
IgY14 LC-10 Column Kit
Includes 1 HPLC column and reagents. |
100 times |
250µl |
SEP040 |
Dig deeper into the proteome with the Seppro IgY Human SuperMix. The SuperMix technology takes your depletion to the next level by further partitioning Moderately Abundant Proteins (MAP).
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