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| Current immunodepletion technology permits rapid processing of plasma samples. However, it still requires significant labor input and has significant throughput limitations. Present plasma biomarker discovery increasingly demands high throughput plasma sample processing. To discover clinically-relevant biomarkers, throughput has to increase to a level of 100 sample processing per day. One of the most important rate-limiting steps in sample processing is depletion of highly-abundant proteins (HAPs). SepproTip platform allows simultaneous processing of 12 samples at a time in a completely automated fashion. Up to 100 plasma or serum samples can be processed daily using the system. The system consists of 12-GC-Pro automation system, disposable pre-filled cartridges, and SepproTip.
Features:
- High throughput. Up to 150 samples/day
- Complete automation. No hands-on manipulations
- Highly cost-effective. Microtips are regenerated automatically and can be re-used for 30 runs
- Buffers come in disposable cartridges, which are loaded for each run
- Highly effective: >96% removal of high-abundant proteins from human serum/plasma samples
Specifications:
- 15 µl of plasma sample/run
- 12 samples per run processing
- 65 minutes per cycle
- Each tip can be used 30 times.
- Each tip is automatically regenerated for next cycle.
Deliverables:
- 12 tips with specific immuno-partitioning media (each tip can be used 30 times):
- IgY14 (removal of top 14 HAPs from human and primate plasma samples) or
- IgY-C12 (removal of top 12 HAPs from cattle plasma/serum samples) or
- IgY-R7 (removal of top 7 HAPs from rat plasma/serum samples) or
- IgY-M7 (removal of top 7 HAPs from mouse plasma/serum samples) or
- IgY-D11 (removal of top 10 HAPs from canine plasma/serum samples) or
- IgY-Rubisco (removal of Rubisco protein from plant protein mixes)
- 432 pre-filled buffer cartridges, containing washing, stripping, neutralization, and regeneration buffers
- 10X Dilution buffer
- 10X Neutralization buffer
- Collection tubes
- 12 GC Magtration Instrument
Recommended Applications:
- Plasma/serum/CSF human biomarker discovery using mass spectrometry
- Plasma/serum/CSF human biomarker discovery using 2D-Gel analysis
- Plasma/serum/CSF mammalian biomarker discovery using mass spectrometry
- Plasma/serum/CSF mammalian biomarker discovery using 2D-gels
- Biomarker validation
- Plant proteomic discovery
Technical Resource:
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