Heterobifunctional Crosslinking Reagents

Heterobifunctional reagents consist of 2 different reactive groups on either end of a spacer arm. These reagents can provide more specific information about the protein interactions and their proximity to one another. This is a result of their ability to perform multiple step cross-linking reactions. In the first step, a protein is modified with one reactive group of the heterobifunctional reagent; the remaining free reagent is removed. In the second step, the modified protein is mixed with a second protein, which is then allowed to react with modifier group at the other end of the reagent. The advantage of having the 2 steps in the conjugation process is that unwanted polymerization or self-linking can be minimized during the free agent removal step.

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B8271 Bromoacetic acid N-hydroxysuccinimide ester ≥95%, powder
M2786 3-Maleimidobenzoic acid N-hydroxysuccinimide ester crystalline
M5525 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester ≥98%, powder
P3415 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester ≥95%, powder