Protease Inhibitor Cocktails

Broad spectrum protease inhibitors and mixtures, or Cocktails, have been developed to help protect the integrity of proteins during the protein extraction and purification process. Sigma manufactures and offers the broadest range of protease inhibitors and inhibitor cocktails available. Our Inhibitor Cocktails have been specifically formulated for particular applications as they relate to the biological source or method of expression.

Individually optimized for:
  • Bacterial cells
  • Mammalian tissues
  • Plant cells
  • HIS-tagged proteins

The full range of Protease Inhibitor Cocktails have been optimized to maintain and preserve protein functionality following cell lysis and are available in powder, solution, or tablet form.

Individual protease inhibitors as well as our offering of non-denaturing cell lysis reagents can be found in our Recombinant Protein Expression Extraction and Stabilization Guide (PDF 268 kb)
See also Enzyme Inhibitors→Protease Inhibitors.

Product #

Product Name

Sample Type

Application

Specificity of Inhibition

Components

Add to Cart
S8830 SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins Optimized and tested for use with HIS-Select media.
 Inhibits serine, cysteine, aspartic, and metalloproteases.
 AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
+ Leupeptin
    • S8820 SIGMAFAST™ Protease Inhibitor Tablets For General Use Optimized and tested for general use.
    		 Inhibits serine, cysteine, aspartic, and metalloproteases.
    		 AEBSF, 2 mM
    Aprotinin, 0.3 μM
    Bestatin, 130 μM
    EDTA, 1 mM
    E-64, 14 μM
    Leupeptin, 1 μM
    • P9599 Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution Tested on extracts from kidney bean, pea, wheat, tobacco, and Arabidopsis.
    		 Inhibits serine, cysteine, aspartic, and metalloproteases, and aminopeptidases.
    		 AEBSF
    Bestatin
    Pepstatin A
    E-64
    Leupeptin
    1,10-Phenanthroline
    • P8849 Protease Inhibitor Cocktail for use in purification of Histidine-tagged proteins, DMSO solution E. coli cells
    baculovirus infected
    Spodoptera frugiperda pupal
    ovary cells
    		 Inhibits serine, cysteine, aspartic and thermolysin-like proteases, and aminopeptidases.
    		 AEBSF
    Bestatin
    E-64
    Pepstatin A
    Phosphoramidon
    • P8465 Protease Inhibitor Cocktail for use with bacterial cell extracts, lyophilized powder Various E. coli strains
    		 Inhibits serine, cysteine, aspartic, Metalloproteases, and Aminopeptidases.
    		 AEBSF, 23 mM
    Bestatin, 2 mM
    EDTA, 100 mM
    E-64, 0.3 mM
    Pepstatin A, 0.3 mM
    • P8340 Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, DMSO solution Use at 1:100 for undiluted blood samples, liver tissue extracts, and CHO cell lysates.
    		 Inhibits, serine, cysteine, aspartic proteases, and amidopeptidases.
    		 AEBSF, 104 mM
    Aprotinin, 80 μM
    Bestatin, 4 mM
    E-64, 1.4 mM
    Leupeptin, 2 mM
    Pepstatin A, 1.5mM
    • P8215 Protease Inhibitor Cocktail for use with fungal and yeast extracts, DMSO solution Saccharomyces cerevisiae cells
    		 Inhibits serine, cysteine, aspartic, and metalloproteases.
    		 AEBSF, 100 mM
    E-64, 1.4 mM
    Pepstatin A, 2.2 mM
    1,10-Phenanthroline, 500mM
    • P2714 Protease Inhibitor Cocktail for general use, lyophilized powder Optimized and tested for general use.
    		 Inhibits serine, cysteine, aspartic, and metalloproteases.
    		 AEBSF, 2 mM
    Aprotinin, 0.3 μM
    Bestatin, 130 μM
    EDTA, 1 mM
    E-64, 14 μM
    Leupeptin, 1 μM
    • P1860 Protease Inhibitor Cocktail for use in tissue culture media, DMSO solution After 48 hours in medium, add fresh inhibitors in fresh medium.
    		 Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
    		 Aprotinin
    Bestatin
    E-64
    Leupeptin
    Pepstatin A
    • I3911 Protease Inhibitor Cocktail, Animal Component Free for general use, lyophilized powder Animal component free (ACF)
    Optimized and tested for general use
    		 Inhibits serine, cysteine, aspartic, and metalloproteases.
    		 AEBSF
    Aprotinin
    Bestatin
    E-64
    EDTA
    Leupeptin
    • I3786 Protease Inhibitor Cocktail, Animal Component Free for use with mammalian cell and tissue extract, DMSO solution Animal component free (ACF)
    		 Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.
    		 Aprotinin
    Bestatin
    E-64
    Leupeptin
    Pepstatin A