The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
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For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation, immunohistochemisty, and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
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Antibody is recommended for use in immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, ELISA, electron microscopy, flow cytometry and supershift assays.
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Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli. Suitable for western blotting.
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