Agarose is the most popular medium for immunoelectrophoresis because of the large pore size for rapid diffusion and for low background staining by Coomassie Blue G stain. The low EEO and high gel strength is specifically selected and tested for immunoelectrophoresis and immunodiffusion.
The most commonly used buffer for immunoelectrophoresis or immunodiffusion of protein on agarose gels or cellulose acetate. Also commonly used for separation of serum proteins, lipoproteins and isoenzymes.
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