These acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Electrophoresis film is a flexible, transparent polyester film specifically designed to support polyacrylamide gels. Ideal for casting horizontal IEF gels. One side has been treated with a hydrophilic coating which enhances adhesion with aqueous polyacrylamide gel media. The acrylamide monomers covalently attach to the coating during polymerization. Gels remain attached during electrophoresis, staining and drying.
Used in denaturing (urea) IEF and two-dimensional electrophoresis.1 Helps facilitate extrusion of the IEF capillary gels from the tubes.2 Used extensively with immobilized pH gradient (IPG) gels.3 Concentrations between 2-4% (v/v) are typically used in an IEF gel.
Detergent used for isoelectric focusing (IEF) and two-dimensional electrophoresis. Concentrations between 1-4% (v/v) are typically used in an IEF gel. Also used for cell permeabilization and protein solubilization including high molecular mass proteins.
A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis. Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
