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Bacterial Cell Lysis

CelLytic B Plus

For efficient protein extraction of Gram+ and Gram- Bacteria

The CelLytic B Plus Kit is designed to efficiently lyse cells and extract proteins (Figure 1) from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.

Features & Benefits

  • Lyse Gram positive and Gram negative bacteria
  • More efficient than sonication
  • Compatible with affinity purification
  • Isolate inclusion bodies for subsequent solubilization
  • Non-denaturing cell lysis preserves protein function

 

Lysis with CelLytic B Plus preserves protein function and is compatible with affinity chromatography (Figure 2). The gentle non-denaturing conditions preserve protein function so that assays can often be performed without removal of lysis reagents. Lysates containing CelLytic B Plus can be applied directly to ANTI-FLAG® M2, and HIS-Select™ Nickel Affinity Gels for direct isolation of tagged recombinant proteins. In addition, insoluble inclusion bodies can be isolated for subsequent solubilization using CelLytic IB and refolding using your method of choice.

Kit Components

  B7435
L3790
E1014
P8849
CelLytic B, Bacterial Lysis Reagent
Lysozyme Solution
Benzonase
Protease Inhibitor Cocktail for Histidine-tagged proteins


Product #  Product Name Package Size
CB0050 CelLytic B Plus Kit: Introductory Size
(Sufficient for 5 g cell paste)
1 kit
CB0500 CelLytic B Plus Kit: Introductory Size
(Sufficient for 50 g cell paste)
1 kit

Figure 1. B. subtilis Extraction, 1 gram of cell paste

Figure 2. CelLytic B Plus kit vs. Leading Competitors
0.5 grams of Bacillus subtilis were lysed using standard procedures. The lysates were then spun to remove cellular debris and the supernatant was analyzed using Bradford Reagent (Prod. Code B6916) and 5 µl of lysate was loaded onto a 4-20% Tris-Glycine Polyacylamide Gel to visualize the proteins. The gel was then stained with EZBlue gel staining reagent (Prod. Code G1041).


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