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Lane 1 - Wide MW Marker (M4038)
Lane 2 - CelLytic Express Lysate
Lane 3 - Column Flow-Through
Lane 4 - Elution
CelLytic Express was used for direct, in-culture lysis of BL21 E. coli cells expressing a Metal Affinity Tag (MAT) protein and cultured in Terrific Broth (T9179). The lysate was then immediately purified using the HIS-Select Nickel Affinity Gel (P6611) by standard methods.
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