RIPA Buffer

RIPA (Radio-Immunoprecipitation Assay) Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins' immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Sigma's RIPA Buffer is a ready-to-use 1X solution and is formulated as follows: 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, and 50 mM Tris, pH 8.0. It is also compatible with EZview™ Red Affinity Gels.

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