CelLytic PN

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves (Figure 1). Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh or frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis, as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and arabidopsis.

Figure 1. Detection of RNA polymerase II in Tomato Nuclear versus Cytoplasmic extracts, prepared with CelLytic PN Kit. The Extracts were run on SDS-PAGE and blot-hybridized to anti-RNA Polymerase II antibody.

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