SigmaFAST™ Protease Inhibitor Cocktail Tablet, EDTA-Free

 Broadest Spectrum Protease Inhibition


SigmaFAST™ Protease Inhibitor Cocktail Tablet, EDTA-Free (Cat. No. S8830) is our water-soluble, convenient protease inhibitor cocktail tablet that protects against:
  • Serine Protease
  • Cysteine Proteases
  • Aspartic Proteases
  • Metalloproteases
Because S8830 is EDTA-free, it does not interfere with downstream applications such as purification of histidine-tagged proteins.


At the onset of tissue or cell lysis, the extract is teeming with proteolytic enzymes, which will degrade proteins. Protecting your proteins from enzymatic degradation is paramount during the extraction and purification processes. It is equally important to have isolated proteins that are functional and compatible with subsequent downstream applications. The development of this tablet provides broad specificity for inhibition of serine, cysteine, metalloprotease, and aspartic (acidic) proteases and can be used for general purposes or for more specialized applications. This cocktail is provided in a convenient water-soluble tablet and offers superior protection without the presence of EDTA chelator or organic solvent, DMSO.

 Compatible with Nickel Resin

Sigma-Aldrich's EDTA-free tablet does not interfere with downstream applications, such as HIS-tag protein purification.

This was tested by repeatedly recovering a HIS-tagged protein from Sigma's HIS-Select Nickel Resin in the presence of the solubilized EDTA-free tablet. As a control, the same HIS-tagged protein was recovered in the presence of PBS (phosphate buffered saline solution). Repeated (4 times) recovery was the same for both sample sets, as measured by protein concentration.

Compatible with Nickel Resin


 Comparison to Competitor

Protease Inhibition using Fluorescent Protease Inhibition Assay.

Protease Inhibition using Fluorescent Protease Inhibition Assay


SDS-PAGE analysis of enzyme digest showing superior protection.

 SDS-PAGE analysis of enzyme digest showing superior protection

A. Control, BSA only
B. BSA, Pepsin Enzyme
C. BSA, Pepsin, SIGMAFAST Protease Inhibitor Cocktail, EDTA-Free Tablet
D. BSA, Pepsin, Competitor EDTA-free Tablet

SDS-PAGE analysis of enzyme digest showing superior protection

A. Control, BSA only
B. BSA, Pancreatin extract
C. BSA, Pancreatin extract, SIGMAFASTä Protease Inhibitor Cocktail, EDTA-Free Tablet


 References

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