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Broadest Spectrum Protease Inhibition Water soluble, convenient EDTA-Free tablet protects against:
And does not interfere downstream applications, such as HIS-tag purification. Compatible with Nickel Resin | Comparison to Competitor | Related Products | Ordering Information At the onset of tissue or cell lysis, the extract is teeming with proteolytic enzymes, which will degrade proteins. Protecting your proteins from enzymatic degradation is paramount during the extraction and purification processes. It is equally important to have isolated proteins that are functional and compatible with subsequent downstream applications. The development of this tablet provides broad specificity for inhibition of serine, cysteine, metalloprotease, and aspartic (acidic) proteases and can be used for general purposes or for more specialized applications. This cocktail is provided in a convenient water-soluble tablet and offers superior protection without the presence of EDTA chelator or organic solvent, DMSO. Sigma-Aldrich's EDTA-free tablet does not interfere with downstream applications, such as HIS-tag protein purification. This was tested by repeatedly recovering a HIS-tagged protein from Sigma's HIS-Select Nickel Resin in the presence of the solubilized EDTA-free tablet. As a control, the same HIS-tagged protein was recovered in the presence of PBS (phosphate buffered saline solution). Repeated (4 times) recovery was the same for both sample sets, as measured by protein concentration.
Protease Inhibition using Fluorescent Protease Inhibition Assay.
SDS-PAGE analysis of enzyme digest showing superior protection. A. Control, BSA only
A. Control, BSA only Protease Inhibitor Cocktails |
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