Yeast Cell Lysis

• CelLytic™ Y
• CelLytic Y Plus Kit

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free, and non-denaturing. It provides an effective method for cell lysis and protein solubilization (Figure 1). Target proteins maintain immunoreactivity or biological function. The protocol is brief and performed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram of yeast cells. Supplement with DTT (5 mM) for enhanced protein yield.

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Sigma's Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immunoreactivity and biological activity.

Kit Components

Reaction buffer 1 M DTT Lyticase (1 ml solution, 25 units/µl) Extraction buffer 10% Triton® X-100 Protease Inhibitor Cocktail

120 ml 5 ml 25,000 units 60 ml 10 ml 2 ml

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