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Life Science > Proteomics > Recombinant Protein Expression > Protocols > MALDI-MS Analysis of Proteins
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Proteomics & Protien Expression
MALDI-MS Analysis of Proteins | ||
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Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. |
View the entire protocol on BioSupplyNet.com.
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Selected Protocol from "Proteins and Proteomics: A Laboratory Manual", Richard J. Simpson. General Method for MALDI-MS Analysis of Proteins and Peptides David Frecklington |
| ABSTRACT | ||
| For MALDI-TOF-MS analysis of proteins and peptides, samples are cocrystallized with an excess of organic matrix that absorbs at a specific wavelength (usually, UV337nm). Typically, sinapinic acid (SA) is the matrix of choice for large proteins, whereas {alpha}-cyano-4-hydroxy-cinnamic acid (HCCA) is the preferred matrix for peptide mapping. Following a short laser pulse, analytes are protonated and desorbed into the gas phase, and their m/z values are determined in a TOF mass analyzer. Mass accuracy determinations vary from ±0.01% to 0.1% depending on the sample preparation technique and the method used for mass calibration. | 
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| Products Available for this Protocol | |||
| Protocol Material Description | Product # | Product Name | Add to Cart |
| Buffers, Solutions, and Reagents | |||
| Acetonitrile | 34967 | Acetonitrile | |
| Methanol | M1775 | Methanol, Absolute - Acetone free | |
| TFA | T6508 | Trifluoroacetic acid, reagent grade | |
| n-octyl glucoside | O8001 | Octyl β-D-glucopyranoside | |
| Standards | Peptide and protein standards for mass spectrometry analysis | ||
| MALDI-MS matrices | Matrices for MALDI Analysis | ||
| Visit the Mass Spectrometry Site for all of your Mass Spec needs. |
| back to top View all available product lists for Cold Spring Harbor Laboratory Protocols |
| Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest. |