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Life Science > Proteomics > Recombinant Protein Expression > Protocols > Preparing GST Fusion Proteins
Proteomics & Protien Expression

Preparing GST Fusion Proteins
cshlp-small Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

View the entire protocol on BioSupplyNet.com.

Selected Protocol from "Protein-Protein Interactions", 2nd Edition, E. Golemis, P. Adam.

Preparation of Glutathione-S-Transferase (GST) Fusion Proteins

Margret B. Einarson and Elena N. Pugacheva
Foxx Chase Cancer Center, Philadelphia, PA 19111

Jason R. Orlinck
Brigham & Women’s Hospital, Boston, MA 02115

ABSTRACT
Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible bacterial expression vectors.   Protein Protein Interactions

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Products Available for this Protocol
Protocol Material Description Product #  Product Name Add to Cart
Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) G4251 L-Glutathione reduced
   
Isopropyl-β-D-thio-galactoside (IPTG) I5502 Isopropyl β-D-1-thiogalactopyranoside
   
LB broth, containing appropriate antibiotic selection L2542 LB Broth
   
PBS P5368 Phosphate buffered saline
   
1% Triton X-100 X-100 Triton® X-100
   
Aprotinin A1153 Aprotinin from bovine lung
   
Leupeptin L9783 Leupeptin hydrochloride
   
Phenylmethylsulfonyl fluoride [PMSF] P7626 Phenylmethanesulfonyl fluoride
   
150 mM NaCl S3014 Sodium chloride
   
20 mM sodium phosphate (pH 7.4) S3264 Sodium phosphate dibasic
   
Glutathione-Sepharose beads G4510 Glutathione−Agarose
   
Coomassie blue (see Sambrook and Russell 2001) B5133 Brilliant Blue G
   
100 mM Tris-HCl (pH 6.8) T3253 Trizma® hydrochloride
   
4%(w/v) SDS (electrophoresis grade) L4390 Sodium dodecyl sulfate
   
0.2% (w/v) bromophenol blue B0126 Bromophenol Blue
   
20% (v/v) glycerol G9012 Glycerol
   
200 mM dithiothreitol (DTT) D9779 DL-Dithiothreitol
   
β-mercaptoethanol M3148 2-Mercaptoethanol
   

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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.