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Proteomics & Protien Expression
Protein Mixtures Mudpit | ||
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Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. |
View the entire protocol on BioSupplyNet.com.
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Selected Protocol from "Proteins and Proteomics: A Laboratory Manual", Richard J. Simpson. Richard J Simpson |
| ABSTRACT | ||
| This protocol describes the analysis of a proteome using multidimensional protein identification technology (MuDPIT), which couples 2D-LC to MS/MS, to resolve and identify peptides from complex mixtures. In this method, a pulled capillary microcolumn is packed with two independent chromatography phases: a strong cation exchanger and reversed-phase matrix material. Once a complex peptide mixture is loaded onto the system, no additional sample handling is required, because, as the peptides elute from the column, they are directed into the ESI ion-trap mass spectrometer, where they are ionized, mass-selected, and fragmented. Finally, advanced search algorithms match the fragmented peptides to their respective proteins in a database. | 
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| Products Available for this Protocol | |||
| Protocol Material Description | Product # | Product Name | Add to Cart |
| Buffers, Solutions, and Reagents | |||
| Acetonitrile | 34967 | Acetonitrile | |
| Ammonium bicarbonate | A6141 | Ammonium bicarbonate | |
| Trypsin | T6567 | Trypsin from porcine pancreas, Proteomics Grade, Dimethylated | |
| Methanol | M1775 | Methanol, Absolute - Acetone free | |
| Dithiothreitol (DTT) | D9779 | DL-Dithiothreitol, for molecular biology | |
| Urea | U5378 | Urea, for molecular biology | |
| Heptafluorobutyric acid | 77247 | Heptafluorobutyric acid | |
| Calcium chloride | 21115 | Calcium chloride solution | |
| Endoproteinase Lys-C | P3428 | Endoproteinase Lys-C from Lysobacter enzymogenes | |
| Iodoacetamide (IAA) | I1149 | Iodoacetamide |
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| Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest. |