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Proteomics
Reversed Phase Microcolumns
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Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. |
View the protocol on BioSupplyNet.com.
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View the protocol, "Preparation of Reversed-Phase Microcolumns," at BioSupplynet.com.
Selected protocol from "Proteins and Proteomics: A Laboratory Manual," Richard J. Simpson.
ABSTRACT
One versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the MALDI target plate. This protocol describes the fabrication of a reversed-phase microcolumn designed for this purpose. The microcolumns are prepared from GELoader tips. This protocol has been optimized for sample cleanup prior to MALDI-MS. However, with slight modifications, it works equally well with samples destined for ESI-MS. |
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Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Chromatography resins |
Chromatography Resins Selection |
| Formic acid |
251364 |
Formic acid, ACS reagent, ≥96.0% |
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| SA (sinapinic acid) |
S8313 |
Sinapinic Acid, Use tested for MALDI-TOF Mass Spec. |
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| DHB (2,5-dihydroxybenzoic acid) |
85707 |
2,5-Dihydroxybenzoic acid, puriss. p.a., matrix substance for MALDI-MS, >99.0% (HPLC) |
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| Acetonitrile |
34998 |
Acetonitrile, CHROMASOLV® Plus, for HPLC, ≥99.9% |
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| CHCA (alpha-cyano-4-hydroxy-cinnamic acid) |
70990 |
α-Cyano-4-hydroxycinnamic acid, puriss. p.a., matrix substance for MALDI-MS, ≥99.0% |
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| TFA (trifluoroacetic acid) |
302031 |
Trifluoroacetic acid, spectrophotometric grade, 99+ % |
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| Methanol |
34860 |
Methanol, CHROMASOLV®, for HPLC, ≥99.9% |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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