| Enterokinase |
| Product No. |
Description
|
Recognition Peptide
|
| E5144 |
Highly specific serine protease used for the removal of the FLAG® peptide from fusion proteins. It is supplied as a Na Cl form, lyophilized from deionized water.
|
LYS-X of FLAG
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-X-C
Note: Peptides are resistant to cleavage if proline occupies position X.
|
| |
| Enterokinase Removal Kit |
| Product No. |
Description
|
Recognition Peptide
|
| PRKE |
Designed as research tool for the removal of bovine enterokinase from mixtures containing a fusion protein cleaved by the enzyme. Using the kit, removal of essentially all enterokinase is accomplished by binding with immobilized rabbit antibodies to calf intestine enterokinase followed by spin filtration.
|
Anti-Enterokinase-Agarose Conjugate, 1.5 mL
20x Wash Buffer, 4 mL
Spin Filters, 10 each
|
| |
| Thrombin CleanCleave™ Kit |
| Product No. |
Description
|
Recognition Peptide
|
| RECOMT |
Bovine thrombin immobilized on 4% beaded agarose designed for cleavage of recombinant fusion proteins. This format circumvents the need for removal of thrombin by chromatographic techniques. The ligand density allows for fast and efficient cleavage; the resin can be reused multiple times with only minimal loss in cleavage efficiency.
|
P4-P3-Pro-Arg/Lys-X-P1'-P2'
Note: P4 and P3 are hydrophobic residues, P1' and P2' are non-acidic residues, and Arg/Lys-X-P1' is the scissile bond.
P2-Arg/Lys-X-P1'
Note: Where P2 or P1' is glycine and Arg/Lys-X-P1' is the scissle bond.
|
| |
| Thrombins |
| Product No. |
Description
|
Recognition Peptide
|
T1063
T7513 |
Thrombin is an endolytic serine protease that selectively cleaves the Arg--Gly bonds. The soluble forms of thrombin are also used for cleavage of recombinant fusion proteins. Human and bovine thrombins have the same peptide recognition sequence.
|
P4-P3-Pro-Arg/Lys-X-P1'-P2'
Note: P4 and P3 are hydrophobic residues, P1' and P2' are non-acidic residues, and Arg/Lys-X-P1' is the scissile bond.
P2-Arg/Lys-X-P1'
Note: Where P2 or P1' is glycine and Arg/Lys-X-P1' is the scissle bond.
|
| F9302 |
Activated Factor X (Xa) is used for cleavage of recombinant fusion protein tags in applications similar to thrombin. Factor Xa catalyzesthe hydrolysis of Arg-Thr and Arg-Ile bonds.
|
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg--X. It maysometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
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