Purification & Detection

FLAG IP Kit

FLAG™ 96-Well Immunoprecipitation Kit (Product Code HT-COIP-1)

The FLAG™-96-Well Immunoprecipitation Kit provides all of the unique reagents required to study protein-protein interactions using a 96-Well ANTI-FLAG™ M2 High Sensitivity Capture Plate (ANTI-FLAG™ M2 plate). The ANTI-FLAG™ M2 plate is designed to capture recombinant FLAG™ fusion proteins obtained from cell lysates of transfected cells.


Reagents Provided (sufficient for 96 samples)
Part Composition Product Code Amount
ANTI-FLAG™ M2 High Sensitivity Capture Plate P 2983 1 plate
Lysis Buffer 20 mM Tris-HCl (pH 7.4), 1 M NaCl, 1 mM DTT, 1.0% Triton® X-100 L 1413 50 ml
10x Wash Buffer 500 mM Tris-HCl (pH 7.4), 1.5 M NaCl W 4511 50 ml
2x Sample Buffer 125 mM Tris-HCl (pH 6.8), 4% SDS, 20% (v/v) glycerol, 0.004% bromphenol blue B 2556 6 ml
Amino-terminal FLAG™-BAP Fusion Protein A 1974 2.5 µg
SealPlate Film Z36,965-9 5