FLAG® System

FLAG® Literature

The FLAG Expression System is the proven and trusted method to express, purify and detect recombinant fusion proteins.

Listed below are many references linked to PubMed, where you can find complete abstract information and relevant journal information.

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  Flag Literature

Fluorescence Microscopy
U2OS GFP-NUP98: Cat. No. CLL1136: U2OS osteosarcoma knock-in cell line expressing the NUP98 gene (nucleoporin 98 kDa, a peripheral component of the NPC) endogenously tagged with 3xFLAG-GFP at the N-terminus.

Click on the title to view the abstract on PubMed.
Title Author / Journal
Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor. Bisson, N.,
Nat Biotechnol. 2011 Jun 26;29(7):
Identifying protein complexes by affinity purification and mass spectrometry analysis in the rice blast fungus. Liu, W.,
Methods Mol Biol. 2011;722:
Hepatitis C virus expressing flag-tagged envelope protein 2 has unaltered infectivity and density, is specifically neutralized by flag antibodies and can be purified by affinity chromatography. Prentoe, J.,
Virology. 2011 Jan 20;409(2):
Biochemical and cellular characterization of Helicobacter pylori RecA, a protein with high-level constitutive expression. Orillard E,
J Bacteriol. 2011 Dec;193(23):
Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes. Fonslow, BR.,
Anal Chem. 2010 Aug 1;82(15):
Herpes simplex virus proteins ICP27 and UL47 associate with polyadenylate-binding protein and control its subcellular distribution. Dobrikova E,
J Virol. 2010 Jan;84(1):
Cell-specific information processing in segregating populations of Eph receptor ephrin-expressing cells. Jørgensen, C.,
Science. 2009 Dec 11;326(5959):
Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo. D'Ambrogio, A.,
Nucleic Acids Res. 2009 Jul;37(12):
Severe acute respiratory syndrome coronavirus papain-like protease ubiquitin-like domain and catalytic domain regulate antagonism of IRF3 and NF-kappaB signaling. Frieman, M.,
J Virol. 2009 Jul;83(13):
Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Wang, Y.,
Mol Cell. 2009 Feb 27;33(4):
Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26. Ofir-Rosenfeld, Y.,
Mol Cell. 2008 Oct 24;32(2):
The protein kinase C-responsive inhibitory domain of CARD11 functions in NF-kappaB activation to regulate the association of multiple signaling cofactors that differentially depend on Bcl10 and MALT1 for association. McCully, RR.,
Mol Cell Biol. 2008 Sep;28(18):
Functional regulation of MyD88-activated interferon regulatory factor 5 by K63-linked polyubiquitination. Balkhi, MY,
Mol Cell Biol. 2008 Dec;28(24):
High-throughput mapping of a dynamic signaling network in mammalian cells. Barrios-Rodiles, M.,
Science. 2005 Mar 11;307(5715):
Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li, FQ., et al.
J.Cell Bio, 2008. 181(7):
Tandem affinity purification vectors for use in gram positive bacteria Yang, X., et al.
Plasmid, 2008. Jan; 59(1): 54-62.
Small-scale production of Burkolderia cepacia ATCC21808 lipase adapted to high-throughput screening Puech-Guenot, S., et al.
Journal of Biomolecular Screening, 2008. Jan; 13(1): 72-79.
Deficient RPS19 protein production induces cell cycle arrest in erythroid progenitor cells Kuramitsu, M., et al.
British Journal of Haematology, 2008. Feb; 140(3): 348-359.
Expression and purification of epitope-tagged multisubunit protein complexes from mammalian cells. Wu, S., et al.
Curr Protoc Mol Biol, 2002. Nov; Chapter 16: Unit 16.13.
Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Murata, T., et al.
Analytical Biochemistry, 2008. Feb 6; 376(1): 115-121.
Proteomic identification of the cerebral cavernous malformation signaling complex Hilder, T. L., et al.
J. Proteome. Res., 2007. Nov; 6(11): 4343-55.
Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels Goetz, J. G., et al.
J. Cell Sci., 2007. Oct 15; 120(Pt 20): 3553-64.
A homogeneous fluorometric assay platform based on novel synthetic proteins Vardar-Schara, G., et al.
Biochem. Biophys. Res. Commun., 2007. Sept 14; 361(1): 103-8.
Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44) Kim, S. H., et al.
Appl. Microbiol. Biotechnol., 2007. Sept; 76(3): 659-65.
Generic method for quantification of FLAG-tagged fusion proteins by a real time biosensor. Mersich, C., et al.
J. Biochem. Biophys. Methods, 2007. Jun 10; 70(4): 555-63.
Expression and purification of recombinant human Angiopoietin-1 produced in Chinese hamster ovary cells Hwang, S. J., et al.
In Vitro Cell. Dev. Biol. - Anim., 2007. May-Jun; 43(5-6): 162-7.
Interaction between Cell Division Proteins FtsE and FtsZ. Corbin, B. D., et al.
J. Bacteriol., 2007. Apr; 189(8): 3026-35.
SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice Giacomodonato, M. N., et al.
Microbiology, 2007. Apr; 153(Pt 4): 1221-8.
Regioselective labeling of antibodies through N-terminal transamination. Scheck, R. A., et al.
ACS Chemical Biol., 2007. April; 2(4): 247-251.
Tyrosine residues 654 and 670 in beta-catenin are crucial in regulation of Met-beta-catenin interactions. Zeng, G., et al.
Exp Cell Res, 2006. Nov 1; 312(18): 3620-30.
Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins. Roosild, T. P., et al.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 2006. Sept 1; 62(9): 835-9.
Design and Characterization of Stimuli-Responsive FLAG-tag Analogues and the Illumination-Induced Modulation of Their Interaction with Antibody 4E11. Pöehner, C., et al.
Biotechnol. Prog., 2006. July-Aug; 22(4): 1170-8.
Construction of eukaryotic expression vector for HPC2 and its expression in HEK293 cells. Tan, K. L., et al.
Di Yi Jun Yi Da Xue Xue Bao, 2005. Dec; 25(12): 1482-1484, 1497.