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FLAG® System

FLAG Literature

The FLAG Expression System is the proven and trusted method to express, purify and detect recombinant fusion proteins.

Listed below are many references linked to PubMed, where you can find complete abstract information and relevant journal information.

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Publications:
Click on the title to view the abstract on PubMed.
Title Author / Journal
Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. Li, FQ., et al.
J.Cell Bio, 2008. 181(7):
1141-1154
Tandem affinity purification vectors for use in gram positive bacteria Yang, X., et al.
Plasmid, 2008. Jan; 59(1): 54-62.
Small-scale production of Burkolderia cepacia ATCC21808 lipase adapted to high-throughput screening Puech-Guenot, S., et al.
Journal of Biomolecular Screening, 2008. Jan; 13(1): 72-79.
Deficient RPS19 protein production induces cell cycle arrest in erythroid progenitor cells Kuramitsu, M., et al.
British Journal of Haematology, 2008. Feb; 140(3): 348-359.
Expression and purification of epitope-tagged multisubunit protein complexes from mammalian cells. Wu, S., et al.
Curr Protoc Mol Biol, 2002. Nov; Chapter 16: Unit 16.13.
Identification of a mitochondrial ATP synthase-adenine nucleotide translocator complex in Leishmania. Detke, S., et al.
Acta Trop, 2008. Jan; 105(1): 16-20.
Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Murata, T., et al.
Analytical Biochemistry, 2008. Feb 6; 376(1): 115-121.
Porcine B-cell activating factor promotes anti-FMDV antibodies in vitro but not in vivo after DNA vaccination of pigs Bergamin, F., et al.
Vet. Immunol. Immunopathol., 2007. Dec 15; 120(3-4): 115-23.
The TATA binding protein associated factor 4b (TAF4b) mediates FSH stimulation of the IGFBP-3 promoter in cultured porcine ovarian granulosa cells Ongeri, E. M., et al.
Mol. Cell. Endocrinol., 2007. Nov 15; 278(1-2): 29-35.
Proteomic identification of the cerebral cavernous malformation signaling complex Hilder, T. L., et al.
J. Proteome. Res., 2007. Nov; 6(11): 4343-55.
Prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide N-acetylgalactosaminyltransferase 14 Wu, C., et al.
Protein Expr. Purif., 2007. Nov; 56(1): 1-7.
Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels Goetz, J. G., et al.
J. Cell Sci., 2007. Oct 15; 120(Pt 20): 3553-64.
Infectivity and neutralization of simian immunodeficiency virus with FLAG epitope insertion in gp120 variable loops Laird, M. E., et al.
J. Virol., 2007. Oct; 81(20): 10838-48.
Delivering genes to the organ-localized immune system: Long-term results of direct intramarrow transduction Louboutin, J. P., et al.
J. Gene Med., 2007. Oct; 9(10): 843-51.
The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney. Fernandes, C. F., et al.
Biochem. Biophys. Res. Commun., 2007. Sept 14; 361(1): 26-32.
A homogeneous fluorometric assay platform based on novel synthetic proteins Vardar-Schara, G., et al.
Biochem. Biophys. Res. Commun., 2007. Sept 14; 361(1): 103-8.
Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44) Kim, S. H., et al.
Appl. Microbiol. Biotechnol., 2007. Sept; 76(3): 659-65.
Human CC ligand 3-like protein 1 (CCL3L1) fusion protein expression and function assay (link to abstract not available) Xu, B., et al.
Zhonghua Weishengwuxue He Mianyixue Zazhi, 2007. Aug; 27(8): 688-92.
Recovery of oligomers and cooperativity when monomers of the M2 muscarinic cholinergic receptor are reconstituted into phospholipid vesicles. Ma, A.W.-S., et al.
Biochemistry, 2007. July 3; 46(26): 7907-7927.
An emerin "Proteome": Purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechanosensing, and nuclear architecture. Holaska, J. M., et al.
Biochemistry, 2007. July 31; 46(30): 8897-908.
An emerin "proteome": purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechanosensing, and nuclear architecture Holaska, J. M., et al.
Biochemistry, 2007. Jul; 46(30): 8897-908.
Generic method for quantification of FLAG-tagged fusion proteins by a real time biosensor. Mersich, C., et al.
J. Biochem. Biophys. Methods, 2007. Jun 10; 70(4): 555-63.
Strategies for CNS-directed gene delivery: in vivo gene transfer to the brain using SV40-derived vectors. Louboutin, J. P., et al.
Gene Ther., 2007. Jun; 14(12): 939-49.
Expression and purification of recombinant human Angiopoietin-1 produced in Chinese hamster ovary cells Hwang, S. J., et al.
In Vitro Cell. Dev. Biol. - Anim., 2007. May-Jun; 43(5-6): 162-7.
BH4 responsiveness associated to a PKU mutation with decreased binding affinity for the cofactor. Aguado, C., et al.
Clinica Chimica Acta, 2007. May 1; 380(1-2): 8-12.
The WW domain protein PRO40 is required for fungal fertility and associates with Woronin bodies. Engh, I., et al.
Eukaryot Cell., 2007. May; 6(5): 831-43.
Risk for infection by lyme disease cause in green surfaces maintenance workers in Belgrade. Krstic, M., et al.
Vojnosanit Pregl, 2007. May; 64(5): 313-8.
Risk for infection by lyme disease cause in green surfaces maintenance workers in Belgrade. Krstic, M., et al.
Vojnosanit Pregl, 2007. May; 64(5): 313-8.
Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver Nath, S., et al.
Mol Biol Cell., 2007. May; 18(5): 1839-49.
S-layer-mediated display of the immunoglobulin G-binding domain of streptococcal protein G on the surface of Caulobacter crescentus: development of an immunoactive reagent Nomellini, J. F., et al.
Appl. Environ. Microbiol., 2007. May; 73(10): 3245-53.
Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica. Tamalampudi, S., et al.
Appl. Microbiol. Biotechnol., 2007. May; 75(2): 387-395.
Follice-stimulating hormone receptor forms oligomers and shows evidence of carboxyl-terminal proteolytic processing Thomas, R. M., et al.
Endocrinology, 2007. May; 148(5): 1987-95.
At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner Unal, R., et al.
J. Neurochem., 2007. May; 101(4): 937-48.
Real-time detection of alpha1A-AR movement stimulated by phenylephrine in single living cells. Xu, N., et al.
Acta Pharmacol. Sin., 2007. May; 28(6): 796-802.
Interaction between Cell Division Proteins FtsE and FtsZ. Corbin, B. D., et al.
J. Bacteriol., 2007. Apr; 189(8): 3026-35.
SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice Giacomodonato, M. N., et al.
Microbiology, 2007. Apr; 153(Pt 4): 1221-8.
Van gogh like-1 protein (Vangl1) a downstream effector of intestinal trefoil factor mediates protection form colitis (link to abstract not available) Kalbis, J., et al.
Gastroenterology, 2007. Apr; 132(4, Suppl. 2): A158.
Regioselective labeling of antibodies through N-terminal transamination. Scheck, R. A., et al.
ACS Chemical Biol., 2007. April; 2(4): 247-251.
Efficient incorporation of a functional hyper-stable single-chain antibody fragment protein-IX fusion in the adenovirus capsid. Vellinga, J., et al.
Gene Therapy, 2007. Apr; 14(8): 664-670.
The Kelch-like protein 1 modulates P/Q-type calcium current density. Aromolaran, K. A,, et al.
Neuroscience, 2007. Mar 30; 145(3): 841-50.
The discovery of new coding alleles of human CYP26A1 that are potentially defective in the metabolism of all-trans retinoic acid and their assessment in a recombinant cDNA expression system. Lee, S. J., et al.
Pharmacogenetics and Genomics, 2007. Mar; 17(3): 169-80.
Intracellular transport and stability of varicella-zoster virus glycoprotein K. Hall, S. L., et al.
Virology, 2007. Feb 20; 358(2): 283-90.
Characterization of capsid-modified adenovirus vectors containing heterologous peptides in the fiber knob, protein IX, or hexon. Kurachi, S., et al.
Gene Therapy, 2007. Feb; 14(3): 266-74.
Mapping of key functions of the herpes simplex virus 1 U(S)3 protein kinase: the U(S)3 protein can form functional heteromultimeric structures derived from overlapping truncated polypeptides. Poon, A. P., et al.
J Virol, 2007. Feb; 81(4): 1980-9.
PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery. Rouleau, M., et al.
J Cell Biochem, 2007. Feb 1; 100(2): 385-401.
LSDP5 is a PAT protein specifically expressed in fatty acid oxidizing tissues. Dalen, K. T., et al.
Biochim Biophys Acta, 2007. Jan; 1771(2): 210-27.
Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. Shukla, A. K., et al.
Int J Biochem Cell Biol, 2007. Jan; 39(5): 931-42.
In vitro phosphorylation and kinase assays in Neurospora crassa. Franchi, L., et al.
Methods Mol. Biol., 2007. 362: 407-412.
Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. Shukla, A. K., et al.
Int J Biochem Cell Biol, 2007. Jan; 39(5): 931-42.
Evidence for the formation of a heptameric ion channel complex by the hepatitis C virus p7 protein in vitro. Clarke, D., et al.
J Biol Chem, 2006. Dec 1; 281(48): 37057-68.
Delivering genes to the organ-localized immune system: Long-term results of direct intramarrow transduction Louboutin, J. P., et al.
J. Gene Med., 2007. Oct; 9(10): 843-51.
Point mutations in the melanocortin-4 receptor cause variable obesity in mice. Meehan, T. P., et al.
Mamm Genome, 2006. Dec; 17(12): 1162-71.
TFF3-peptide increases transepithelial resistance in epithelial cells by modulating claudin-1 and -2 expression. Meyer zum Bueschenfelde, D., et al.
Peptides, 2006. Dec; 27(12): 3383-3390.
Secretory expression of synthetic human Fas ligand extracellular domain gene in Pichia pastoris: influences of tag addition and N-glycosylation site deletion, and development of a purification method. Muraki, M.
Protein Expr Purif, 2006. Dec; 50(2): 137-46.
HSP70 binds to SHP2 and has effects on the SHP2-related EGFR/GAB1 signaling pathway. Yoo, J. C., et al.
Biochem Biophys Res Commun, 2006. Dec 29; 351(4): 979-85.
Tyrosine residues 654 and 670 in beta-catenin are crucial in regulation of Met-beta-catenin interactions. Zeng, G., et al.
Exp Cell Res, 2006. Nov 1; 312(18): 3620-30.
Human Immunodeficiency Virus-Type 1 LTR DNA contains an intrinsic gene producing antisense RNA and protein products. Ludwig, L. B., et al.
Retrovirology, 2006. Nov 8; 3(80).
Antibody binding is a dominant determinant of the efficiency of human immunodeficiency virus type 1 neutralization Xang, X., et al.
J. Virol., 2006. Nov; 80(22): 11404-8.
A platform of high-efficiency nonviral gene transfer in mouse osteoblast cells in vitro Xing, W., et al.
Mol. Biotechnol., 2006. Sept; 34(1): 29-36.
Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins. Roosild, T. P., et al.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 2006. Sept 1; 62(9): 835-9.
G protein-coupled receptor 30 is an estrogen receptor in the plasma membrane. Funakoshi, T., et al.
Biochem Biophys Res Commun, 2006. Aug 4; 346(3): 904-10.
Characterization of the Pilin Ortholog of the Helicobacter pylori Type IV cag Pathogenicity Apparatus, a Surface-Associated Protein Expressed during Infection. Andrzejewska, J., et al.
J Bacteriol, 2006. Aug; 188(16): 5865-77.
Design and Characterization of Stimuli-Responsive FLAG-tag Analogues and the Illumination-Induced Modulation of Their Interaction with Antibody 4E11. Pöehner, C., et al.
Biotechnol. Prog., 2006. July-Aug; 22(4): 1170-8.
Nitroglycerin attenuates human endothelial progenitor cell function, differentiation and survival. DiFabio, J. M., et al.
J Pharmacol Exp Ther, 2006. July; 318(1): 117-23.
Flash labeling of a nuclear receptor domain (D domain of ultraspiracle) fused to tetracysteine tag. Szécsi, M., et al.
Acta Biol Hung, 2006. Jun; 57(2): 181-90.
Phosphorylation of paracellin-1 at Ser217 by protein kinase A is essential for localization in tight junctions. Ikari, A., et al.
J Cell Sci, 2006. May 1; 119(9): 1781-9.
A two-event model of transfusion-related acute lung injury. Silliman, C. C., et al.
Crit Care Med, 2006. May; 34(5 Suppl): S124-31.
Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity. Fuchs, H. C., et al.
Clin Exp Allergy, 2006. Mar; 36(3): 359-68.
Membrane Topology of Human ASBT (SLC10A2) Determined by Dual Label Epitope Insertion Scanning Mutagenesis. New Evidence for Seven Transmembrane Domains. Banerjee, A., et al.
Biochemistry, 2006. Jan 24; 45(3): 943-53.
PKCI-W forms a heterodimer with PKCI-Z and inhibits the biological activities of PKCI-Z in vitro, supporting the predicted role of PKCI-W in sex determination in birds. Moriyama, S., et al.
J Biochem (Tokyo), 2006. Jan; 139(1): 91-7.
PKCI-W forms a heterodimer with PKCI-Z and inhibits the biological activities of PKCI-Z in vitro, supporting the predicted role of PKCI-W in sex determination in birds. Moriyama, S., et al.
J Biochem (Tokyo), 2006. Jan; 139(1): 91-7.
Construction of eukaryotic expression vector for HPC2 and its expression in HEK293 cells. Tan, K. L., et al.
Di Yi Jun Yi Da Xue Xue Bao, 2005. Dec; 25(12): 1482-1484, 1497.
An activin-A/C chimera exhibits activin and myostatin antagonistic properties Muenster, U., et al.
J. Biol. Chem., 2005. Nov 4; 280(44): 36626-32.
Quantitative atomic force microscopy image analysis of unusual filaments formed by the Acanthamoeba castellanii myosin II rod domain Rigotti, D. J., et al.
Anal. Biochem., 2005. Nov 15; 346(2): 189-200.
High-throughput screening for inhibitors of the e3 ubiquitin ligase APC. Huang, J., et al.
Methods Enzymol, 2005. 399: 740-54.
Two variants of the rat brain sodium-driven chloride bicarbonate exchanger (NCBE): developmental expression and addition of a PDZ motif. Giffard, R. G., et al.
Eur J Neurosci, 2003. Dec; 18(11): 2935-45.
Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9) and in HEK293 cells. Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer. Gazi, L., et al.
Eur. J. Biochem., 2003. Oct; 270(19): 3928-38.
Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70. Nuttall, S. D., et al.
Eur. J. Biochem., 2003. Sep; 270(17): 3543-54.
Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Kimura, M., et al.
Mol. Cell. Biol., 2002. Mar; 22(5): 1577-88.
Development of a robust scintillation proximity assay for protein tyrosine phosphatase 1B using the catalytically inactive (C215S) mutant. Skorey, K. I., et al.
Anal Biochem, 2001. Apr 15; 291(2): 269-78.
Biosynthesis of inositol trisphosphate receptors: selective association with the molecular chaperone calnexin. Joseph, S. K., et al.
Biochem J., 1999. Aug 15; 342(Pt 1): 153-61.
Topology of subunit a of the Escherichia coli ATP synthase. Joager, H., et al.
Eur. J. Biochem., 1998. Jan 15; 251(1-2): 122-32.
Molecular cloning of the hamster CMP-sialic acid transporter. Eckhardt, M., et al.
Eur. J. Biochem., 1997. Aug 15; 248(1): 187-92.
Olfactory receptor proteins. Expression, characterization and partial purification. Gat, U., et al.
Eur. J. Biochem., 1994. Nov 1; 225(3): 1157-68.


 
 
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