HIS-Select® Technology

Sigma's HIS-Select products have the ability to purify histidine-tagged proteins quickly and with high selectivity. This is due to HIS-Select's patented, non-charged, hydrophilic nickel chelate linkage. This non-charged linkage greatly diminishes non-specific binding. Most other systems for histidine-tagged protein purification utilize a charged chelate linkage. The tetradentate chelator used in the HIS-Select products results in greatly reduced nickel leaching and in high specificity for purification or affinity capture of your histidine-tagged protein. In addition, HIS-Select eliminates the need for costly and time-consuming secondary purification of your protein, allowing for One-step-purification.
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High Selectivity for Higher Purity Non-charged, hydrophilic linkage reduces non-specific binding Pure tetradentate chelate for minimal metal leaching One-step purification

Nickel Affinity Gel High Flow Nickel Affinity Gel HIS-Select® Cobalt Affinity Gel IMAC-Select Affinity Gel	HIS-Select iLAP® 5 ml Column Cartridge Spin Column EZview™ Red Nickel Affinity Gel	High Capacity Nickel Coated Plates High Sensitivity Nickel Coated Plates HIS-Select® Filter Plates
M Purification Kit Y Purification Kit M Affinity Capture Kit  Wash & Elution Buffer Kit	Trypsin  Maldi Kits CelLytics ProteoQwest Kits ProteoSilver HIS-Select® Wash & Elution Buffers

• HIS-Select^® Quick Reference Guide
• Highly Selective HIS-Select^® Requires Much Less Imidazole

Recent R&D Posters:

• High Capacity Capture of Histidine-Tagged Recombinant Proteins
• Affinity Capture Resins With Enhanced Visibility
• High Throughput Platform Suitable for Common Proteomic Techniques

Life Science Quarterly Application Note:

• HIS-Select® Affinity Gels and Plates for Affinity Capture of Histidine-Containing Proteins

Automation Protocols:

• Automation Protocol for HIS-Select® iLAP® Plates

Additional Literature:

• Protein Expression & Analysis Product Guide

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HIS-Select and products are covered by US Patent No 6,623,655 and licensed under U.S. Patent No. 4,569,794 and are sold for research use only. Commercial use of Sigma's HIS-Select, PHOS-Select, iLAP and IMAC products require additional licenses.